Document Detail

MEK/ERK pathway mediates UVB-induced AQP1 downregulation and water permeability impairment in human retinal pigment epithelial cells.
MedLine Citation:
PMID:  19424603     Owner:  NLM     Status:  MEDLINE    
Aquaporins (AQPs) are a family of 13 small ( approximately 30 kDa/monomer), hydrophobic, integral membrane proteins. AQPs are expressed in various epithelial and endothelial cells involved in fluid transport. Here, we demonstrated for the first time that AQP1 is expressed in cultured human retinal pigment epithelial (RPE) cells (ARPE-19 cell line). Ultraviolet radiation (UVB) and H2O2, two major factors causing RPE cell damage, induced AQP1 downregulation which was mediated by MEK/ERK activation. UV and H2O2 as well as AQP1-specific siRNA knockdown impaired water permeability of ARPE-19 cells. Notably, pretreatment with all-trans retinoic acid attenuated UV- and H2O2-induced AQP1 downregulation and water permeability impairment. Considering that water permeability is involved in multiple functions of RPE cells such as cellular junction formation, fluid or protein exchange and barrier formation, our data elucidated a novel mechanism through which UV radiation and oxidative stress induce eye cell damage. Our results further support the notion that all-trans retinoic acid might be useful for protection against UV or oxidative stress-induced eye cell damage.
Qin Jiang; Cong Cao; Shan Lu; Rebecca Kivlin; Brittany Wallin; Wenming Chu; Zhigang Bi; Xinru Wang; Yinsheng Wan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of molecular medicine     Volume:  23     ISSN:  1107-3756     ISO Abbreviation:  Int. J. Mol. Med.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-08     Completed Date:  2009-07-30     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9810955     Medline TA:  Int J Mol Med     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  771-7     Citation Subset:  IM    
Eye Hospital, Nanjing Medical University, Nanjing 210029, People's Republic of China.
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MeSH Terms
Aquaporin 1 / genetics,  metabolism*
Blotting, Western
Cell Line
Cell Membrane Permeability / drug effects,  radiation effects*
Down-Regulation / drug effects,  genetics,  radiation effects*
Hydrogen Peroxide / pharmacology
Mitogen-Activated Protein Kinase Kinases / metabolism*
Pigment Epithelium of Eye / cytology*
RNA, Small Interfering / genetics,  physiology
Reactive Oxygen Species / metabolism
Tretinoin / pharmacology
Ultraviolet Rays*
Water / metabolism*
Grant Support
Reg. No./Substance:
0/RNA, Small Interfering; 0/Reactive Oxygen Species; 146410-94-8/Aquaporin 1; 302-79-4/Tretinoin; 7722-84-1/Hydrogen Peroxide; 7732-18-5/Water; EC Protein Kinase Kinases

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