Document Detail


Pathogenic potential of Campylobacter ureolyticus.
MedLine Citation:
PMID:  22124656     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The recent detection and isolation of the aflagellate Campylobacter ureolyticus (previously known as Bacteroides ureolyticus) from intestinal biopsy specimens and fecal samples of children with newly diagnosed Crohn's disease led us to investigate the pathogenic potential of this bacterium. Adherence and gentamicin protection assays were employed to quantify the levels of adherence to and invasion into host cells. C. ureolyticus UNSWCD was able to adhere to the Caco-2 intestinal epithelial cell line with a value of 5.341% ± 0.74% but was not able to invade the Caco-2 cells. The addition of two proinflammatory cytokines, tumor necrosis factor alpha (TNF-α) and gamma interferon (IFN-γ), to the cell line did not affect attachment or invasion, with attachment levels being 4.156% ± 0.61% (P = 0.270) for TNF-α and 6.472% ± 0.61% (P = 0.235) for IFN-γ. Scanning electron microscopy visually confirmed attachment and revealed that C. ureolyticus UNSWCD colonizes and adheres to intestinal cells, inducing cellular damage and microvillus degradation. Purification and identification of the C. ureolyticus UNSWCD secretome detected a total of 111 proteins, from which 29 were bioinformatically predicted to be secretory proteins. Functional classification revealed three putative virulence and colonization factors: the surface antigen CjaA, an outer membrane fibronectin binding protein, and an S-layer RTX toxin. These results suggest that C. ureolyticus has the potential to be a pathogen of the gastrointestinal tract.
Authors:
Jose A Burgos-Portugal; Nadeem O Kaakoush; Mark J Raftery; Hazel M Mitchell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-11-28
Journal Detail:
Title:  Infection and immunity     Volume:  80     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-23     Completed Date:  2012-03-27     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  883-90     Citation Subset:  IM    
Affiliation:
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, Australia.
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents
Bacterial Adhesion
Bacterial Proteins / genetics,  metabolism
Caco-2 Cells
Campylobacter / classification,  pathogenicity*
Campylobacter Infections / microbiology*
Computational Biology
Gastrointestinal Diseases / microbiology
Gene Expression Regulation, Bacterial / physiology
Gentamicins / pharmacology
Humans
Interferon-gamma
Microscopy, Electron, Scanning
Tumor Necrosis Factor-alpha
Virulence
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bacterial Proteins; 0/Gentamicins; 0/Tumor Necrosis Factor-alpha; 82115-62-6/Interferon-gamma
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