| A passively operated microfluidic device for stimulation and secretion sampling of single pancreatic islets. | |
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MedLine Citation:
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PMID: 21806019 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A passively operated polydimethylsiloxane (PDMS) microfluidic device was designed for sampling of hormone secretions from eight individual murine pancreatic islets in parallel. Flow control was achieved using a single hand-held syringe and by exploiting inherent fluidic resistances of the microchannels (R<sub>sampling</sub> = 700 ± 20 kPa s mm<sup>-3</sup> at 37 °C). Basal (3 mM) or stimulatory (11 mM) glucose levels were applied to islets, with stimulation timing (t<sub>stim</sub>) minimized to 15 ± 2 s using modified reservoirs. Using enzyme-linked immunosorbent assays (ELISA) for post-sampling analyses, we measured statistically equal levels of one-hour insulin secretion (1.26 ± 0.26 and 6.55 ± 1.00 pg islet<sup>-1</sup> min<sup>-1</sup>, basal and stimulated; 62 islets) compared to standard, bulk sampling methods (1.01 ± 0.224 and 6.04 ± 1.53 pg islet<sup>-1</sup> min<sup>-1</sup>, basal and stimulated; 200 islets). Importantly, the microfluidic platform revealed novel information on single-islet variability. Islet volume measurements with confocal reflectance microscopy revealed that insulin secretion had only limited correlation to islet volume, suggesting a more significant role for cellular architecture and paracrine signaling within the tissue. Compared to other methods using syringe pumps or electroosmotic flow control, this approach provides significant advantages in ease-of-use and device disposability, easing the burden on non-experts. |
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Authors:
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Leah A Godwin; Meagan E Pilkerton; Kennon S Deal; Desiree Wanders; Robert L Judd; Christopher J Easley |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-8-1 |
Journal Detail:
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Title: Analytical chemistry Volume: - ISSN: 1520-6882 ISO Abbreviation: - Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-8-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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