Document Detail


Matriptase/epithin participates in mammary epithelial cell growth and morphogenesis through HGF activation.
MedLine Citation:
PMID:  19853659     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The epithelial-derived, type II transmembrane serine protease matriptase, the mouse homologue of which is epithin, has been shown to be involved in epidermal differentiation, hair formation, and thymus function. We show in this study that epithin/matriptase (Epi/MTP) plays a significant role in mammary epithelial cell growth and morphogenesis. Epi/MTP is expressed at low level in the mouse mammary epithelium of young animals and it accumulates at the terminal end-bud of the growing ducts. The level of Epi/MTP is elevated in the mammary glands at stages when epithelial proliferation and modeling occur. It is primarily present in the luminal epithelial cells of mouse mammary ducts and lobules. Using an ex vivo three-dimensional culture system for mammary epithelial functional assays, we show that mammary epithelial growth and morphogenesis in the presence of the latent form hepatocyte growth factor (pro-HGF) are blocked either by an inhibitor of the Epi/MTP protease activity or by siRNA knockdown of the Epi/MTP expression. These studies demonstrate that Epi/MTP participates in mammary epithelial growth and modeling through activation of pro-HGF. Our findings reveal an important pathway in normal mammary epithelial morphogenesis which may participate in breast cancer progression.
Authors:
Sheau-Ling Lee; Pao-Yi Huang; Peter Roller; Eun-Gyung Cho; Dongeun Park; Robert B Dickson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-22
Journal Detail:
Title:  Mechanisms of development     Volume:  127     ISSN:  1872-6356     ISO Abbreviation:  Mech. Dev.     Publication Date:    2010 Jan-Feb
Date Detail:
Created Date:  2010-02-03     Completed Date:  2010-05-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9101218     Medline TA:  Mech Dev     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  82-95     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Institute of Cellular and Systems Medicine, National Health Research Institutes, Taiwan, ROC. sllee@nhri.org.tw
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques / methods
Cell Proliferation
Dose-Response Relationship, Drug
Gene Expression Regulation, Developmental*
Hepatocyte Growth Factor / metabolism*
Mammary Glands, Animal / metabolism
Mammary Neoplasms, Animal / metabolism
Mice
Microscopy, Confocal / methods
Peptides, Cyclic / metabolism
RNA Interference
RNA, Small Interfering / metabolism
Serine Endopeptidases / metabolism,  physiology*
Time Factors
Chemical
Reg. No./Substance:
0/Peptides, Cyclic; 0/RNA, Small Interfering; 0/SFTI-1 peptide, sunflower; 67256-21-7/Hepatocyte Growth Factor; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.-/matriptase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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