Document Detail

A parametric study of freezing injury in BPH1CAFTD-2 human prostate tumor cells.
MedLine Citation:
PMID:  17898905     Owner:  NLM     Status:  MEDLINE    
Freeze injury in BPH1(CAFTD)-2 cells frozen/thawed in suspension was studied through a two-level four-parameter (2(4)) experimental design and analysis. The four parameters considered were end temperature, hold time, TNFalpha concentration, and thawing rate. Thermal parameter values chosen were based on the approximate thermal history cells would experience in the peripheral region of a cryosurgical iceball. Cell suspensions were frozen at a constant 10 degree C/min on a directional solidification stage and viability was assessed within 1 hr post-thaw using a dye exclusion assay. The parameters affecting cell survival were determined through calculation of the individual parameter effects (E) and interactions (I) according to factorial design guidelines; data set curvature (C) was also determined. Cell viability ranged from a maximum of 87.6 percent to a minimum of 17.6 percent indicating trends in cell survival were sensitive to the parameters chosen. Survival was affected by the following parameters in order: lowering the end temperature, increasing the hold time, adding TNFalpha, and reducing the thawing rate. In addition, all 2-way parameter interactions except TNFalpha hold time were statistically significant. Curvature analysis showed that cell viability at the midpoint of the data was nearly 20 percent lower than predicted based on linear interpolation. These results were verified and extended using analysis of variance (ANOVA). We conclude that cryoinjury in this tumor line can be influenced by multiple interacting thermal parameters, most importantly end-temperature and hold time, as well as the presence of the cytokine TNFalpha. Finally, although the cell type is tumorigenic results suggest the possibility of using freezing to control benign prostatic hyperplasia (BPH) in addition to cancer within the prostate.
Matthew G Geeslin; David J Swanlund; John C Bischof
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryo letters     Volume:  28     ISSN:  0143-2044     ISO Abbreviation:  Cryo Letters     Publication Date:    2007 May-Jun
Date Detail:
Created Date:  2007-09-27     Completed Date:  2007-10-24     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  9891832     Medline TA:  Cryo Letters     Country:  England    
Other Details:
Languages:  eng     Pagination:  173-86     Citation Subset:  IM    
Department of Mechanical Engineering,university of Minnesota, Minneappolis, Minnesota 55455, USA.
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MeSH Terms
Analysis of Variance
Cell Line, Tumor / drug effects
Cell Survival
Drug Administration Schedule
Prostatic Neoplasms / pathology,  physiopathology*
Time Factors
Tumor Necrosis Factor-alpha / administration & dosage,  pharmacology
Grant Support
Reg. No./Substance:
0/Tumor Necrosis Factor-alpha

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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