Document Detail

A parametric study of freezing injury in ELT-3 uterine leiomyoma tumour cells.
MedLine Citation:
PMID:  11157831     Owner:  NLM     Status:  MEDLINE    
Cellular level freeze injury was investigated after controlled freezing of an Eker rat uterine fibroid cell line in both the presence and absence of oestradiol. The connection between thermal history and cell injury in single ELT-3 cells in suspension (without oestradiol) was studied through a two-level, four-parameter (2(4)) experiment with membrane dye exclusion as the end-point. The four parameters considered were cooling rate (CR), end temperature (ET), hold time (HT) and thawing rate (TR). A high and low value of each parameter was selected as follows: CR, 5-25 degrees C/min; ET, -20 to -30 degrees C; HT, 0-5 min; TR 20-200 degrees C/min. The greatest parameter effect on freeze injury in this range was ET followed by HT, then TR and finally CR. In addition, significant parameter interactions and curvature were found. Additional CR results outside the original parameter range showed a reduction in survival at both 1 and 50 degrees C/min suggestive of an inverted U-shaped survival curve. These results show that this tumour system is susceptible to cryoinjury, particularly at temperatures below -30 degrees C with HT of >5 min and slow thawing. In addition, the presence of oestradiol was found to increase the susceptibility of these cells to cryoinjury.
J Bischof; W Fahssi; D Smith; T Nagel; D Swanlund
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  16     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-05-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  340-8     Citation Subset:  IM    
Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN 55455, USA.
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MeSH Terms
Cell Survival / drug effects
Estradiol / pharmacology
Leiomyoma / pathology,  surgery*
Tumor Cells, Cultured
Uterine Neoplasms / pathology,  surgery*
Reg. No./Substance:

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