Document Detail


A palmitoyl-CoA-specific delta9 fatty acid desaturase from Caenorhabditis elegans.
MedLine Citation:
PMID:  10872837     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biosynthesis of polyunsaturated fatty acids in C. elegans is initiated by the introduction of a double bond at the delta9 position of a saturated fatty acid. We identified three C. elegans fatty acid desaturase genes related to the yeast delta9 desaturase OLE1 and the rat stearoyl-CoA desaturase SCD1. Heterologous expression of all three genes rescues the fatty acid auxotrophy of the yeast delta9 desaturase mutant ole1. Examination of the fatty acid composition of the transgenic yeast reveals striking differences in the substrate specificities of these desaturases. Two desaturases, FAT-6 and FAT-7, readily desaturate stearic acid (18:0) and show less activity on palmitic acid (16:0). In contrast, the other desaturase, FAT-5, readily desaturates palmitic acid (16:0), but shows nearly undetectable activity on the common delta9 substrate stearic acid. This is the first report of a palmitoyl-CoA-specific membrane fatty acid desaturase.
Authors:
J L Watts; J Browse
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  272     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-07-17     Completed Date:  2000-07-17     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  263-9     Citation Subset:  IM    
Affiliation:
Institute of Biological Chemistry, Washington State University, Pullman 99164-6340, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Caenorhabditis elegans / enzymology*,  genetics*
Cloning, Molecular
DNA Primers / genetics
Fatty Acid Desaturases / genetics*,  metabolism*
Genes, Helminth
Molecular Sequence Data
Rats
Saccharomyces cerevisiae / enzymology,  genetics
Sequence Homology, Amino Acid
Chemical
Reg. No./Substance:
0/DNA Primers; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.99.-/delta-9 fatty acid desaturase; EC 1.14.99.-/palmitoyl-CoA desaturase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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