Document Detail

A palmitoyl-CoA-specific delta9 fatty acid desaturase from Caenorhabditis elegans.
MedLine Citation:
PMID:  10872837     Owner:  NLM     Status:  MEDLINE    
Biosynthesis of polyunsaturated fatty acids in C. elegans is initiated by the introduction of a double bond at the delta9 position of a saturated fatty acid. We identified three C. elegans fatty acid desaturase genes related to the yeast delta9 desaturase OLE1 and the rat stearoyl-CoA desaturase SCD1. Heterologous expression of all three genes rescues the fatty acid auxotrophy of the yeast delta9 desaturase mutant ole1. Examination of the fatty acid composition of the transgenic yeast reveals striking differences in the substrate specificities of these desaturases. Two desaturases, FAT-6 and FAT-7, readily desaturate stearic acid (18:0) and show less activity on palmitic acid (16:0). In contrast, the other desaturase, FAT-5, readily desaturates palmitic acid (16:0), but shows nearly undetectable activity on the common delta9 substrate stearic acid. This is the first report of a palmitoyl-CoA-specific membrane fatty acid desaturase.
J L Watts; J Browse
Related Documents :
16014477 - Roseovarius crassostreae sp. nov., a member of the roseobacter clade and the apparent c...
7203877 - High-pressure liquid chromatography of fatty acid esters of retinol isomers. analysis o...
23419767 - Abdominal adiposity is associated with fatty acid desaturase activity in boys: implicat...
1556107 - Specificity of unsaturated fatty acid-regulated expression of the saccharomyces cerevis...
24276127 - Geranium defensive agents. iii. structural determination and biosynthetic consideration...
51407 - Liver damage after paracetamol overdose. comparison of liver-function tests, fasting se...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  272     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-07-17     Completed Date:  2000-07-17     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  263-9     Citation Subset:  IM    
Institute of Biological Chemistry, Washington State University, Pullman 99164-6340, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acid Sequence
Base Sequence
Caenorhabditis elegans / enzymology*,  genetics*
Cloning, Molecular
DNA Primers / genetics
Fatty Acid Desaturases / genetics*,  metabolism*
Genes, Helminth
Molecular Sequence Data
Saccharomyces cerevisiae / enzymology,  genetics
Sequence Homology, Amino Acid
Reg. No./Substance:
0/DNA Primers; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.99.-/delta-9 fatty acid desaturase; EC 1.14.99.-/palmitoyl-CoA desaturase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Chimeric synthetic peptide as antigen for immunodiagnosis of HIV-1 infection.
Next Document:  Identification of a 26S proteasome-associated UCH in fission yeast.