| A palmitoyl-CoA-specific delta9 fatty acid desaturase from Caenorhabditis elegans. | |
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MedLine Citation:
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PMID: 10872837 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Biosynthesis of polyunsaturated fatty acids in C. elegans is initiated by the introduction of a double bond at the delta9 position of a saturated fatty acid. We identified three C. elegans fatty acid desaturase genes related to the yeast delta9 desaturase OLE1 and the rat stearoyl-CoA desaturase SCD1. Heterologous expression of all three genes rescues the fatty acid auxotrophy of the yeast delta9 desaturase mutant ole1. Examination of the fatty acid composition of the transgenic yeast reveals striking differences in the substrate specificities of these desaturases. Two desaturases, FAT-6 and FAT-7, readily desaturate stearic acid (18:0) and show less activity on palmitic acid (16:0). In contrast, the other desaturase, FAT-5, readily desaturates palmitic acid (16:0), but shows nearly undetectable activity on the common delta9 substrate stearic acid. This is the first report of a palmitoyl-CoA-specific membrane fatty acid desaturase. |
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Authors:
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J L Watts; J Browse |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 272 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2000 May |
Date Detail:
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Created Date: 2000-07-17 Completed Date: 2000-07-17 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 263-9 Citation Subset: IM |
Affiliation:
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Institute of Biological Chemistry, Washington State University, Pullman 99164-6340, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Caenorhabditis elegans / enzymology*, genetics* Cloning, Molecular DNA Primers / genetics Fatty Acid Desaturases / genetics*, metabolism* Genes, Helminth Molecular Sequence Data Rats Saccharomyces cerevisiae / enzymology, genetics Sequence Homology, Amino Acid |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.99.-/delta-9 fatty acid desaturase; EC 1.14.99.-/palmitoyl-CoA desaturase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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