Document Detail


pHMA, a pH-sensitive GFP reporter for cell engulfment, in Drosophila embryos, tissues, and cells.
MedLine Citation:
PMID:  20014104     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Engulfment of apoptotic cells by phagocytosis ensures the removal of unwanted and defective cells. We developed a genetically encoded marker for cell engulfment, pHMA, which consists of the pH-Sensitive derivative of GFP, pHluorin, fused to the actin-binding domain of Moesin. In healthy cells of Drosophila embryos and cultured cells, pHMA resides at the cell cortex. In dying cells, pHMA loses its cortical localization and reports a modest decrease in pH. In embryos, the dying cells lose their apical contacts, then move basally and are ultimately engulfed by neighboring cells or macrophages. The cell corpse material is strongly acidified soon after engulfment and persists in the phagocytic cell for several hours. Changes in the pHMA signal correlate well with increases or decreases in apoptosis. These data show that pHMA is a useful reporter for cell engulfment and can be used in screening for mutations that affect cell engulfment.
Authors:
Elane Fishilevich; James A J Fitzpatrick; Jonathan S Minden
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  239     ISSN:  1097-0177     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-05-04     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  559-73     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA.
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MeSH Terms
Descriptor/Qualifier:
Acridine Orange
Animals
Apoptosis*
Autophagy
Cell Line
Drosophila
Genes, Reporter*
Green Fluorescent Proteins / genetics,  metabolism*
Hydrogen-Ion Concentration
Larva / metabolism
Microfilament Proteins / genetics,  metabolism*
Phagocytosis*
Protein Interaction Domains and Motifs
Grant Support
ID/Acronym/Agency:
1R21 HD049554/HD/NICHD NIH HHS; 5U54 RR022241/RR/NCRR NIH HHS; R21 HD049554-01A1/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Microfilament Proteins; 0/PHluorin; 144131-77-1/moesin; 147336-22-9/Green Fluorescent Proteins; 65-61-2/Acridine Orange
Comments/Corrections

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