| pHH3 Immunostaining Improves Interobserver Agreement of Mitotic Index in Thin Melanomas. | |
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MedLine Citation:
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PMID: 22197861 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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According to the seventh edition of the American Joint Committee on Cancer guidelines, the TNM staging category in thin cutaneous melanomas depends on the mitotic rate (MR). In this study, we analyze the interobserver agreement of the MR in a series of 92 thin cutaneous melanomas. Serial sections of the tumors were either stained with hematoxylin and eosin or immunohistochemically stained with pHH3, an antibody for phosphohistone H3, and analyzed by 4 observers. Determination of MR with pHH3 immunostaining resulted in higher sensitivity in counting mitosis for all observers. Moreover, interobserver agreement was higher with pHH3. Immunostaining with pHH3 is a sensitive method to detect mitosis in thin cutaneous melanomas, with good reproducibility of MR between independent observers. Further studies are needed to find out if higher sensitivity in the detection of mitosis by pHH3 immunostaining has additional prognostic relevance. |
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Authors:
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Tobias T Schimming; Florian Grabellus; Mathias Roner; Sonali Pechlivanis; Antje Sucker; Nicola Bielefeld; Iris Moll; Dirk Schadendorf; Uwe Hillen |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-22 |
Journal Detail:
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Title: The American Journal of dermatopathology Volume: - ISSN: 1533-0311 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7911005 Medline TA: Am J Dermatopathol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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From the Departments of *Dermatology, Venerology and Allergology; †Pathology and Neuropathology; and ‡Medical Informatics, Biometry and Epidemiology, University Duisburg-Essen, Essen, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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