| pH-zone-refining countercurrent chromatography. | |
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MedLine Citation:
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PMID: 8962503 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A recently developed preparative technique, pH-zone-refining countercurrent chromatography (CCC), separates organic acids and bases according to their pKa and hydrophobicity. The hydrodynamic mechanism of pH-zone-refining CCC is illustrated in two elution modes along with a simple mathematical analysis. Separations include acidic and basic derivatives of amino acids and peptides, hydroxyxanthene dyes, alkaloids, indole auxins, and structural and geometrical isomers. In addition, recently developed affinity ligand separations of enantiomers, polar catecholamines, and free peptides are also described. Technical guidance is provided for interested users so that they can conduct a systematic search for the optimum solvent system and experimental conditions. Advantages as well as limitations of the present technique are discussed. |
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Authors:
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Y Ito; Y Ma |
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Publication Detail:
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Type: Journal Article; Review |
Journal Detail:
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Title: Journal of chromatography. A Volume: 753 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 1996 Nov |
Date Detail:
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Created Date: 1997-01-16 Completed Date: 1997-01-16 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 1-36 Citation Subset: IM |
Affiliation:
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Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1676, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Countercurrent Distribution* Hydrogen-Ion Concentration Models, Theoretical |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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