Document Detail

The pH volume changes of human red cells in vitro due to the exchange of chloride and hydroxyl anions.
MedLine Citation:
PMID:  1298578     Owner:  NLM     Status:  MEDLINE    
In glucose exit experiments measured photoelectrically, the excursions on the chart recorder were found to be larger for exits in media of alkaline pH. This was shown to be due to the addition of a pH volume effect to that of the osmotic shrinkage resulting from the glucose efflux. The pH-dependent volume change also occurred in glucose-free cells and was a linear function of the pH of the medium between pH 6.8 and 9.0. The effect is consistent with the loss (or gain) of chloride in exchange for hydroxyl anions on the band 3 anion transporter and with the buffering of the hydroxyl anions by haemoglobin. The implications for the working of the anion exchanger and for respiratory physiology are discussed.
W F Widdas; G F Baker
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Cytobios     Volume:  72     ISSN:  0011-4529     ISO Abbreviation:  Cytobios     Publication Date:  1992  
Date Detail:
Created Date:  1993-05-12     Completed Date:  1993-05-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0207227     Medline TA:  Cytobios     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  139-52     Citation Subset:  IM    
Department of Biology (Physiology), Royal Holloway and Bedford New College, University of London, Egham, Surrey, Great Britain.
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MeSH Terms
Anion Exchange Protein 1, Erythrocyte / metabolism*
Cell Size / physiology
Chlorides / blood*
Culture Media / pharmacology
Erythrocytes / cytology*,  drug effects,  metabolism
Hydrogen-Ion Concentration*
Hydroxides / blood*
Osmotic Pressure
Respiration / physiology
Reg. No./Substance:
0/Anion Exchange Protein 1, Erythrocyte; 0/Chlorides; 0/Culture Media; 0/Hydroxides; 14280-30-9/hydroxide ion

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