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pH responsive PEGylation through metal affinity for gene delivery mediated by histidine-grafted polyethylenimine.
MedLine Citation:
PMID:  23165821     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Polyethylene glycol (PEG) has been used to enhance the stability of a gene delivery system. The most commonly used approach is to add the PEG molecule by way of chemical conjugation. In this study, we prepared PEG-bearing nitrilotriacetic acid (ntaPEG) followed by chelation with either nickel or zinc ions. Polyethylenimine was grafted with histidine (hisPEI) and used as a primary gene carrier to form complexes with DNA. PEGylation was performed by incubating the complexes with chelated ntaPEG. It was noted that the coating of the chelated ntaPEG could provide a shielding effect against aggregation induced by bovine serum albumin and DNA release induced by heparin displacement, respectively. The coating was also found to improve cellular viability and maintain the transfection efficiency at a moderate level. The coated ntaPEG could dissociate from the complexes in an acidic condition of pH 4, suggesting that dePEGylation might occur in some acidic intracellular organelles, such as endosomes. This simple and effective PEGylation approach could be extended to other delivery systems to enhance the stability and to facilitate the dePEGylation process. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.
Authors:
Wen-Chi Tseng; Ling-Yu Su; Tsuei-Yun Fang
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-19
Journal Detail:
Title:  Journal of biomedical materials research. Part B, Applied biomaterials     Volume:  -     ISSN:  1552-4981     ISO Abbreviation:  J. Biomed. Mater. Res. Part B Appl. Biomater.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234238     Medline TA:  J Biomed Mater Res B Appl Biomater     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan. tsengwc@mail.ntust.edu.tw.
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