| A pH-dependent structural transition in the homopurine-homopyrimidine tract in superhelical DNA. | |
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MedLine Citation:
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PMID: 3917024 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have inserted the 509-bp-long fragment of sea urchin P. miliaris histone gene spacer region into plasmid pUC19. The fragment contains the 60-bp-long homopurine-homopyrimidine tract that is known to be hypersensitive to the S1 endonuclease. Using two-dimensional gel electrophoresis we have observed a sharp structural transition in the insert with increasing DNA superhelicity. As in the cases of cruciform and Z form formation, the observed transition partly relaxes the superhelical stress. In contrast with the other two well documented transitions, the observed transition strongly depends on pH. At pH7 and above the transition occurs at negative superhelicities exceeding the physiological range (- sigma greater than 0.08). For pH6 the transition occurs at -sigma = 0.055, whereas for pH4.3 it takes place at -sigma = 0.001. A comprehensive analysis of the obtained data has made it possible to define the nature of the observed transition. We conclude that under superhelical stress or/and at low pH homopurinehomopyrimidine tracts adopt a novel spatial structure called the H form. |
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Authors:
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V I Lyamichev; S M Mirkin; M D Frank-Kamenetskii |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biomolecular structure & dynamics Volume: 3 ISSN: 0739-1102 ISO Abbreviation: J. Biomol. Struct. Dyn. Publication Date: 1985 Oct |
Date Detail:
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Created Date: 1990-03-07 Completed Date: 1990-03-07 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8404176 Medline TA: J Biomol Struct Dyn Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 327-38 Citation Subset: IM |
Affiliation:
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Institute of Molecular Genetics, USSR Academy of Sciences, Moscow. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence DNA, Superhelical* / genetics Histones / genetics Hydrogen-Ion Concentration Models, Chemical Molecular Sequence Data Nucleic Acid Conformation* Sea Urchins Single-Strand Specific DNA and RNA Endonucleases |
| Chemical | |
Reg. No./Substance:
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0/DNA, Superhelical; 0/Histones; EC 3.1.30.1/Single-Strand Specific DNA and RNA Endonucleases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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