| pH-dependent Uptake of Fumaric Acid in Saccharomyces cerevisiae under Anaerobic Conditions. | |
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MedLine Citation:
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PMID: 22113915 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Microbial production of C4-dicarboxylic acids from renewable resources has gained renewed interest. The yeast Saccharomyces cerevisiae is known as a robust microorganism and able to grow at low pH, which makes it a suitable candidate for biological production of organic acids. However, a successful metabolic engineering approach for overproduction of organic acids requires an incorporation of a proper exporter to increase the productivity. Moreover, low pH fermentations, which are desirable for facilitating the downstream processing may cause back diffusion of the undissociated acid into the cells with simultaneous active export, thereby creating an ATP dissipating futile cycle. In this work we have studied the uptake of fumaric acid in S. cerevisiae in carbon limited chemostat cultures under anaerobic conditions. The effect of the presence of fumaric acid at different pH values (3 - 5) has been investigated in order to obtain more knowledge about possible uptake mechanisms. The experimental results showed that at a cultivation pH 5.0 and an external fumaric acid concentration of approx. 0.8 mmol.L(-1) the fumaric acid uptake rate was unexpectedly high, and could not be explained by diffusion of the undissociated form across the plasma membrane alone. This would indicate the presence of protein mediated import. At decreasing pH the fumaric acid uptake rate was found to increase asymptotically to a maximum level. Although also this observation is in accordance with protein mediated import, the presence of a metabolic bottleneck for fumaric acid conversion under anaerobic conditions could not be excluded. |
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Authors:
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Elaheh Jamalzadeh; Peter J T Verheijen; Joseph J Heijnen; Walter M van Gulik |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-23 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: - ISSN: 1098-5336 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Biotechnology, Kluyver Centre for Genomics of Industrial Fermentation, Delft University of Technology, Delft, The Netherlands. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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