Document Detail


pH control strategy in a shaken minibioreactor for polysaccharide production by medicinal mushroom Phellinus linteus and its anti-hyperlipemia activity.
MedLine Citation:
PMID:  18587601     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A process at various pH values ranging from 4.5 to 7.5 for production of mycelia and extracellular polysaccharide (EPS) by P. linteus fermentation in pH-controlled shaken bioreactor was investigated. A two-stage pH control strategy in which pH value was kept at 6.5 for the first 24 h, and then switched to 4.5 was developed successfully to enhance simultaneously the cell growth and EPS production. The maximum cell density and EPS production reached 15.13 +/- 0.1 g/l on day 6 and 6.74 +/- 0.1 g/l on day 4, respectively. The anti- hyperlipemia effect of EPS and intracellular polysaccharide (IPS) extracted from mycelia were observed that both EPS and IPS can obviously reduce the serum triglyceride (TG), the blood cholesterol (TC) and serum low density lipoprotein (LDL) level, and increase the high density lipoprotein (HDL) level of the hyperlipemia mice. Polysaccharides from submerged cultivation of medicinal fungus P. linteus have favorable potency to develop anti-hyperlipermia drugs.
Authors:
Xiang Zou; Xia Guo; Min Sun
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-28
Journal Detail:
Title:  Bioprocess and biosystems engineering     Volume:  32     ISSN:  1615-7605     ISO Abbreviation:  Bioprocess Biosyst Eng     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-19     Completed Date:  2009-03-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088505     Medline TA:  Bioprocess Biosyst Eng     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  277-81     Citation Subset:  IM    
Affiliation:
School of Pharmaceutical Sciences, Southwest University, 400715 Chongqing, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Agaricales / metabolism*
Bioreactors*
Fermentation
Hydrogen-Ion Concentration*
Hyperlipidemias / drug therapy*
Polysaccharides / biosynthesis*,  therapeutic use
Chemical
Reg. No./Substance:
0/Polysaccharides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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