Document Detail

pH control in rat skeletal muscle during exercise, recovery from exercise, and acute respiratory acidosis.
MedLine Citation:
PMID:  8133746     Owner:  NLM     Status:  MEDLINE    
We used 31P magnetic resonance spectroscopy to compare the response of rat skeletal muscle to three kinds of proton load. During exercise (tetanic sciatic nerve stimulation), protons from lactic acid were buffered passively and consumed by net hydrolysis of phosphocreatine (PCr). During recovery from exercise, the pH-dependent efflux of protons produced by PCr resynthesis could be partially inhibited by amiloride or 4,4'-diisothiocyanostilbene-2,2'-disulphonate (DIDS), implicating both sodium/proton and bicarbonate/chloride exchange, but was not inhibited by simultaneous respiratory acidosis. In early recovery, up to 30% of proton efflux was mediated by lactate/proton cotransport. During acute respiratory acidosis at rest, the eventual change in muscle pH was consistent with passive buffering and was unaffected by amiloride or DIDS, implying no significant contribution of proton fluxes.
G J Kemp; C H Thompson; A L Sanderson; G K Radda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine     Volume:  31     ISSN:  0740-3194     ISO Abbreviation:  Magn Reson Med     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-04-21     Completed Date:  1994-04-21     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8505245     Medline TA:  Magn Reson Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  103-9     Citation Subset:  IM; S    
MRC Biochemical and Clinical Magnetic Resonance Unit, John Radcliffe Hospital, Oxford, United Kingdom.
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MeSH Terms
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / pharmacology
Acidosis, Respiratory / blood,  metabolism,  physiopathology*
Acute Disease
Adenosine Diphosphate / metabolism
Amiloride / pharmacology
Carbon Dioxide / blood
Carbonates / metabolism
Electric Stimulation
Hydrogen-Ion Concentration
Lactates / biosynthesis
Lactic Acid
Magnetic Resonance Spectroscopy / diagnostic use
Muscles / drug effects,  metabolism,  physiology*
Phosphocreatine / metabolism
Physical Exertion / physiology*
Rats, Wistar
Rest / physiology
Sciatic Nerve / physiology
Reg. No./Substance:
0/Buffers; 0/Carbonates; 0/Lactates; 124-38-9/Carbon Dioxide; 2609-46-3/Amiloride; 50-21-5/Lactic Acid; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 58-64-0/Adenosine Diphosphate; 67-07-2/Phosphocreatine; 7723-14-0/Phosphorus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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