| pH control in rat skeletal muscle during exercise, recovery from exercise, and acute respiratory acidosis. | |
MedLine Citation:
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PMID: 8133746 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We used 31P magnetic resonance spectroscopy to compare the response of rat skeletal muscle to three kinds of proton load. During exercise (tetanic sciatic nerve stimulation), protons from lactic acid were buffered passively and consumed by net hydrolysis of phosphocreatine (PCr). During recovery from exercise, the pH-dependent efflux of protons produced by PCr resynthesis could be partially inhibited by amiloride or 4,4'-diisothiocyanostilbene-2,2'-disulphonate (DIDS), implicating both sodium/proton and bicarbonate/chloride exchange, but was not inhibited by simultaneous respiratory acidosis. In early recovery, up to 30% of proton efflux was mediated by lactate/proton cotransport. During acute respiratory acidosis at rest, the eventual change in muscle pH was consistent with passive buffering and was unaffected by amiloride or DIDS, implying no significant contribution of proton fluxes. |
Authors:
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G J Kemp; C H Thompson; A L Sanderson; G K Radda |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine Volume: 31 ISSN: 0740-3194 ISO Abbreviation: Magn Reson Med Publication Date: 1994 Feb |
Date Detail:
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Created Date: 1994-04-21 Completed Date: 1994-04-21 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8505245 Medline TA: Magn Reson Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 103-9 Citation Subset: IM; S |
Affiliation:
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MRC Biochemical and Clinical Magnetic Resonance Unit, John Radcliffe Hospital, Oxford, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
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pharmacology Acidosis, Respiratory / blood, metabolism, physiopathology* Acute Disease Adenosine Diphosphate / metabolism Amiloride / pharmacology Animals Buffers Carbon Dioxide / blood Carbonates / metabolism Electric Stimulation Hydrogen-Ion Concentration Lactates / biosynthesis Lactic Acid Magnetic Resonance Spectroscopy / diagnostic use Muscles / drug effects, metabolism, physiology* Phosphocreatine / metabolism Phosphorus Physical Exertion / physiology* Rats Rats, Wistar Rest / physiology Sciatic Nerve / physiology |
| Chemical | |
Reg. No./Substance:
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0/Buffers; 0/Carbonates; 0/Lactates; 124-38-9/Carbon Dioxide; 2609-46-3/Amiloride; 50-21-5/Lactic Acid; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 58-64-0/Adenosine Diphosphate; 67-07-2/Phosphocreatine; 7723-14-0/Phosphorus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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