Document Detail

pEYFP-Nuc vector is a useful tool for three-dimensional and time-lapse observation of nuclear morphology of Jurkat cells during apoptosis.
MedLine Citation:
PMID:  14719129     Owner:  NLM     Status:  MEDLINE    
Using a pEYFP-Nuc vector, which contains nucleic acid sequences of a nuclear localization signal, we established a Jurkat-YN cell line that expressed enhanced yellow fluorescent protein (EYFP) in the nucleus. We observed three-dimensional and time-lapse changes in nuclear morphology of Jurkat-YN cells during Fas-induced apoptosis using a confocal laser scanning microscope. The nuclear forms visualized by EYFP were almost equal in quality to those visualized by SYTO59, a nucleic acid stain for living cells. Three-dimensional deformities in the nuclear form were observed during apoptosis before chromatin condensation became apparent, indicating these deformities are characteristic morphological changes of the early stage of apoptosis. In conclusion, the pEYFP-Nuc vector is a useful tool in the time-lapse observation of nuclear morphology of living cells during apoptosis.
Akira Hasegawa; Masahiro Kuroda; Yadi Wang; Shiro Akaki; Jun-Ichi Asaumi; Kohichi Shibuya; Shoji Kawasaki; Yoshihiro Okumura; Susumu Kanazawa; Yoshio Hiraki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of molecular medicine     Volume:  13     ISSN:  1107-3756     ISO Abbreviation:  Int. J. Mol. Med.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-01-13     Completed Date:  2004-03-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9810955     Medline TA:  Int J Mol Med     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  235-42     Citation Subset:  IM    
Department of Radiology, Okayama University Medical School, Okayama 700-8558, Japan.
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MeSH Terms
Antibodies, Monoclonal / immunology
Antigens, CD95 / immunology
Apoptosis / immunology,  physiology*
Cell Nucleus / immunology,  physiology*
Genes, Reporter*
Genetic Vectors*
Jurkat Cells / cytology*
Microscopy, Confocal
Microscopy, Fluorescence
Time Factors
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD95

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