Document Detail


p63 induces CD4+ T-cell chemoattractant TARC/CCL17 in human epithelial cells.
MedLine Citation:
PMID:  18937546     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To preserve immunosurveillance, epithelial cells support T-cell trafficking toward inflammatory foci. However, how epithelial cells are enrolled in recruiting T cells has not been fully elucidated. In this study we investigated the function of p63, a p53 family member, in the regulation of the expression of various types of chemokine ligands by focusing on the property of p63 as an epitheliotropic transcription factor. As assessed by experiments using three different human epithelial cell lines with small-interfering RNAs or plasmids of p63, certain CC chemokine ligands were found to be under the control of p63. In these CC chemokine ligands, p63 had the common capacity to upregulate TARC/CCL17 in the different cell lines, whose receptor CCR4 was preferentially presented on CD4(+) T cells such as memory, regulatory, IL-17-producing and type II helper T cells. More interestingly, when cells were stimulated with transforming growth factor-beta (TGF-beta) or epidermal growth factor (EGF) as observed during tissue repair process, the expression of p63 and TARC/CCL17 was concomitantly suppressed. This implies that, in local inflammatory regions with general epithelial tissue remodeling, the p63-TARC/CCL17 axis may participate in the engagement of efficient immune reactions by specified T-cell subsets.
Authors:
Terufumi Kubo; Shingo Ichimiya; Akiko Tonooka; Tsutomu Nagashima; Tomoki Kikuchi; Noriyuki Sato
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research     Volume:  28     ISSN:  1557-7465     ISO Abbreviation:  J. Interferon Cytokine Res.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-01     Completed Date:  2009-09-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9507088     Medline TA:  J Interferon Cytokine Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  725-32     Citation Subset:  IM    
Affiliation:
Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
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MeSH Terms
Descriptor/Qualifier:
CD4-Positive T-Lymphocytes / immunology*,  metabolism
Cell Line
Chemokine CCL17 / immunology,  metabolism*
Epidermal Growth Factor / immunology,  metabolism
Epithelial Cells / cytology,  immunology*,  metabolism
Humans
Membrane Proteins / immunology,  metabolism*
Receptors, CXCR / immunology,  metabolism*
Transcription, Genetic
Transforming Growth Factor beta / immunology,  metabolism
Chemical
Reg. No./Substance:
0/CCL17 protein, human; 0/CKAP4 protein, human; 0/Chemokine CCL17; 0/Membrane Proteins; 0/Receptors, CXCR; 0/Transforming Growth Factor beta; 62229-50-9/Epidermal Growth Factor

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