Document Detail

p63 expression in solid cell nests of the thyroid: further evidence for a stem cell origin.
MedLine Citation:
PMID:  12527712     Owner:  NLM     Status:  MEDLINE    
Solid cell nests of the thyroid are embryonic remnants of endodermal origin that may be difficult to distinguish from squamous metaplasia, metastatic squamous carcinoma, papillary microcarcinoma, medullary carcinoma, and C-cell hyperplasia. These embryonic structures are composed of main cells and C-cells; cystic structures and mixed follicles are sometimes observed intermingled with solid cell nests. Recently, p63, a p53 homologue that is consistently expressed in basal/stem cells of stratified epithelia and plays a major role in triggering the differentiation of some specific cell lineages, has been characterized. We evaluated the immunohistochemical expression of p63, cytokeratins (CAM 5.2, AE1/AE3, 34betaE12, 7, and 20), carcinoembryonic antigen, thyroid transcription factor 1 (TTF-1), thyroglobulin, and calcitonin using the streptavidin-biotin-peroxidase complex technique in 6 bona fide solid cell nests. We observed that main cells of solid cell nests are strongly decorated by p63, while C-cells and all other thyroid structures were consistently negative. Moreover, main cells expressed carcinoembryonic antigen and all cytokeratins but cytokeratin 20 and lacked TTF-1, thyroglobulin and calcitonin. In contrast to this, C-cells of solid cell nests were immunoreactive for calcitonin, CAM 5.2, AE1/AE3, and cytokeratin 7; focal immunoreactivity for TTF-1 was also observed in some C-cells. We conclude that main cells of the solid cell nests display a basal/stem cell phenotype (p63 and basal cytokeratin positivity), whereas C-cells show features of parafollicular differentiation. We conclude, furthermore, that p63 antibodies may help in distinguishing solid cell nests from their mimics.
Jorge S Reis-Filho; Ana Preto; Paula Soares; Sara Ricardo; José Cameselle-Teijeiro; Manuel Sobrinho-Simões
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc     Volume:  16     ISSN:  0893-3952     ISO Abbreviation:  Mod. Pathol.     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2003-01-15     Completed Date:  2003-07-10     Revised Date:  2008-06-27    
Medline Journal Info:
Nlm Unique ID:  8806605     Medline TA:  Mod Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  43-8     Citation Subset:  IM    
Institute of Molecular Pathology and Immunology, University of Porto, Porto, Portugal.
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MeSH Terms
Adenoma / metabolism*,  pathology
Carcinoembryonic Antigen / metabolism
Carcinoma, Papillary / metabolism*,  pathology
DNA-Binding Proteins
Genes, Tumor Suppressor
Immunoenzyme Techniques
Keratins / metabolism
Membrane Proteins*
Middle Aged
Phosphoproteins / metabolism*
Stem Cells / metabolism*,  pathology
Thyroid Neoplasms / metabolism*,  pathology
Trans-Activators / metabolism*
Tumor Markers, Biological / metabolism
Tumor Suppressor Proteins
Reg. No./Substance:
0/CKAP4 protein, human; 0/Carcinoembryonic Antigen; 0/DNA-Binding Proteins; 0/Membrane Proteins; 0/Phosphoproteins; 0/TP63 protein, human; 0/Trans-Activators; 0/Tumor Markers, Biological; 0/Tumor Suppressor Proteins; 68238-35-7/Keratins

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