| p63 expression in solid cell nests of the thyroid: further evidence for a stem cell origin. | |
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MedLine Citation:
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PMID: 12527712 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Solid cell nests of the thyroid are embryonic remnants of endodermal origin that may be difficult to distinguish from squamous metaplasia, metastatic squamous carcinoma, papillary microcarcinoma, medullary carcinoma, and C-cell hyperplasia. These embryonic structures are composed of main cells and C-cells; cystic structures and mixed follicles are sometimes observed intermingled with solid cell nests. Recently, p63, a p53 homologue that is consistently expressed in basal/stem cells of stratified epithelia and plays a major role in triggering the differentiation of some specific cell lineages, has been characterized. We evaluated the immunohistochemical expression of p63, cytokeratins (CAM 5.2, AE1/AE3, 34betaE12, 7, and 20), carcinoembryonic antigen, thyroid transcription factor 1 (TTF-1), thyroglobulin, and calcitonin using the streptavidin-biotin-peroxidase complex technique in 6 bona fide solid cell nests. We observed that main cells of solid cell nests are strongly decorated by p63, while C-cells and all other thyroid structures were consistently negative. Moreover, main cells expressed carcinoembryonic antigen and all cytokeratins but cytokeratin 20 and lacked TTF-1, thyroglobulin and calcitonin. In contrast to this, C-cells of solid cell nests were immunoreactive for calcitonin, CAM 5.2, AE1/AE3, and cytokeratin 7; focal immunoreactivity for TTF-1 was also observed in some C-cells. We conclude that main cells of the solid cell nests display a basal/stem cell phenotype (p63 and basal cytokeratin positivity), whereas C-cells show features of parafollicular differentiation. We conclude, furthermore, that p63 antibodies may help in distinguishing solid cell nests from their mimics. |
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Authors:
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Jorge S Reis-Filho; Ana Preto; Paula Soares; Sara Ricardo; José Cameselle-Teijeiro; Manuel Sobrinho-Simões |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc Volume: 16 ISSN: 0893-3952 ISO Abbreviation: Mod. Pathol. Publication Date: 2003 Jan |
Date Detail:
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Created Date: 2003-01-15 Completed Date: 2003-07-10 Revised Date: 2008-06-27 |
Medline Journal Info:
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Nlm Unique ID: 8806605 Medline TA: Mod Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 43-8 Citation Subset: IM |
Affiliation:
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Institute of Molecular Pathology and Immunology, University of Porto, Porto, Portugal. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenoma
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metabolism*,
pathology Adult Aged Carcinoembryonic Antigen / metabolism Carcinoma, Papillary / metabolism*, pathology DNA-Binding Proteins Female Genes, Tumor Suppressor Humans Immunoenzyme Techniques Keratins / metabolism Male Membrane Proteins* Middle Aged Phosphoproteins / metabolism* Stem Cells / metabolism*, pathology Thyroid Neoplasms / metabolism*, pathology Trans-Activators / metabolism* Tumor Markers, Biological / metabolism Tumor Suppressor Proteins |
| Chemical | |
Reg. No./Substance:
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0/CKAP4 protein, human; 0/Carcinoembryonic Antigen; 0/DNA-Binding Proteins; 0/Membrane Proteins; 0/Phosphoproteins; 0/TP63 protein, human; 0/Trans-Activators; 0/Tumor Markers, Biological; 0/Tumor Suppressor Proteins; 68238-35-7/Keratins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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