Document Detail

p62 and NDP52 proteins target intracytosolic Shigella and Listeria to different autophagy pathways.
MedLine Citation:
PMID:  21646350     Owner:  NLM     Status:  MEDLINE    
Autophagy is an important mechanism of innate immune defense. We have recently shown that autophagy components are recruited with septins, a new and increasingly characterized cytoskeleton component, to intracytosolic Shigella that have started to polymerize actin. On the other hand, intracytosolic Listeria avoids autophagy recognition by expressing ActA, a bacterial effector required for actin polymerization. Here, we exploit Shigella and Listeria as intracytosolic tools to characterize different pathways of selective autophagy. We show that the ubiquitin-binding adaptor proteins p62 and NDP52 target Shigella to an autophagy pathway dependent upon septin and actin. In contrast, p62 or NDP52 targets the Listeria ActA mutant to an autophagy pathway independent of septin or actin. TNF-α, a host cytokine produced upon bacterial infection, stimulates p62-mediated autophagic activity and restricts the survival of Shigella and the Listeria ActA mutant. These data provide a new molecular framework to understand the emerging complexity of autophagy and its ability to achieve specific clearance of intracytosolic bacteria.
Serge Mostowy; Vanessa Sancho-Shimizu; Mélanie Anne Hamon; Roxane Simeone; Roland Brosch; Terje Johansen; Pascale Cossart
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-06-06
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  286     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-07-25     Completed Date:  2011-09-28     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  26987-95     Citation Subset:  IM    
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MeSH Terms
Actins / genetics,  metabolism
Adaptor Proteins, Signal Transducing / genetics,  metabolism*
Bacterial Proteins
Cytosol / metabolism,  microbiology
Dysentery, Bacillary / genetics,  metabolism*
HeLa Cells
Listeria monocytogenes / genetics,  metabolism*
Listeriosis / genetics,  metabolism*
Membrane Proteins
Nuclear Proteins / genetics,  metabolism*
Septins / genetics,  metabolism
Shigella flexneri / genetics,  metabolism*
Tumor Necrosis Factor-alpha / genetics,  metabolism
Grant Support
233348//European Research Council; //Howard Hughes Medical Institute
Reg. No./Substance:
0/Actins; 0/Adaptor Proteins, Signal Transducing; 0/Bacterial Proteins; 0/Membrane Proteins; 0/Nuclear Proteins; 0/SQSTM1 protein, human; 0/Tumor Necrosis Factor-alpha; 0/nuclear dot protein 52, human; 144430-05-7/actA protein, Listeria monocytogenes; EC 3.6.1.-/Septins

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