Document Detail


p53 arrests growth and induces differentiation of v-Myb-transformed monoblasts.
MedLine Citation:
PMID:  17309603     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The p53 protein can control cell cycle progression, programmed cell death, and differentiation of many cell types. Ectopic expression of p53 can resume capability of cell cycle arrest, differentiation, and apoptosis in various leukemic cell lines. In this work, we expressed human p53 protein in v-Myb-transformed chicken monoblasts. We found that even this protein possessing only 53% amino acid homology to its avian counterpart can significantly alter morphology and physiology of these cells causing the G2-phase cell cycle arrest and early monocytic differentiation. Our results document that the species-specific differences of the p53 molecules, promoters/enhancers, and co-factors in avian and human cells do not interfere with differentiation- and cell cycle arrest promoting capabilites of the p53 tumor suppressor even in the presence of functional v-Myb oncoprotein. The p53-induced differentiation and cell cycle arrest of v-Myb-transformed monoblasts are not associated with apoptosis suggesting that the p53-driven pathways controlling apoptosis and differentiation/proliferation are independent.
Authors:
Jarmila Navrátilová; Viktor Horváth; Alois Kozubík; Antonín Lojek; Joseph Lipsick; Jan Smarda
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-02-16
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  75     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-29     Completed Date:  2007-10-30     Revised Date:  2013-01-17    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  England    
Other Details:
Languages:  eng     Pagination:  592-604     Citation Subset:  IM    
Affiliation:
Institute of Experimental Biology, Faculty of Science, Masaryk University, Kotlárská 2, 611 37 Brno, Czech Republic.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / genetics
Cell Cycle / genetics
Cell Differentiation / genetics,  physiology*
Cell Line, Transformed
Cell Proliferation*
Chickens
G2 Phase / genetics
Growth Inhibitors / genetics,  physiology*
Humans
Monocytes / cytology*
Oncogene Proteins v-myb / genetics*
Signal Transduction / genetics
Transfection
Tumor Suppressor Protein p53 / genetics,  physiology*
Grant Support
ID/Acronym/Agency:
R01 CA128836/CA/NCI NIH HHS; R01 CA43592/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Growth Inhibitors; 0/Oncogene Proteins v-myb; 0/Tumor Suppressor Protein p53

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