| p38-Targeted inhibition of interleukin-12 expression by ethanol extract from Cordyceps bassiana in lipopolysaccharide-activated macrophages. | |
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MedLine Citation:
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PMID: 20476843 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Cordyceps species have been known as ethnopharmacologically valuable mushroom in Korea, China, and Japan. This plant has been reported to exhibit a variety of pharmacological activities such as antioxidative, anticancer, anti-inflammatory, antidiabetic, and antiobesity effects. Although numerous pharmacological potentials of Cordyceps spp. have been demonstrated, immunomodulatory effect of Cordyceps bassiana has not been published yet. To evaluate its immunomodulatory activity, macrophages activated by lipopolysaccharide (LPS) were employed and the production of interleukin-12 (IL-12) was explored in terms of understanding its molecular inhibitory mechanism. Seventy percent of ethanol extract from Cordyceps bassiana (Cb-EE) was able to suppress the expression of IL-12, a cytokine regulating interferon-γ (IFN-γ)-producing T helper type 1 (Th1) polarization response, at the transcriptional levels. The inhibitory effect of Cb-EE seemed to be due to activator protein-1 (AP-1) translocation inhibition, according to immunoblotting analysis with nuclear fraction and luciferase assay. In agreement with this, Cb-EE strongly suppressed the phosphorylation of p38, a prime signal to stimulate AP-1 translocation and IL-12 production, strongly suppressed by SB203580, a p38 inhibitor. Furthermore, this extract also suppressed IFN-γ production in both phytohemaglutinin A and LPS-activated splenocytes. Our results suggest that Cb-EE can be applied as a Th1 response regulatory herbal medicine. |
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Authors:
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Se Eun Byeon; Jaehwi Lee; Byong Chul Yoo; Gi Ho Sung; Tae Woong Kim; Hyoung Jin Park; Jae Youl Cho |
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Publication Detail:
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Type: Journal Article Date: 2010-05-17 |
Journal Detail:
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Title: Immunopharmacology and immunotoxicology Volume: 33 ISSN: 1532-2513 ISO Abbreviation: Immunopharmacol Immunotoxicol Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-02-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8800150 Medline TA: Immunopharmacol Immunotoxicol Country: England |
Other Details:
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Languages: eng Pagination: 90-6 Citation Subset: IM |
Affiliation:
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School of Bioscience and Biotechnology, and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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