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p27(kip1) Upregulated by hnRNPC1/2 Antagonizes CagA (a Virulence Factor of Helicobacter pylori)-Mediated Pathogenesis.
MedLine Citation:
PMID:  22404445     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Background and Aims:  Infection by Helicobacter pylori is one of the major contributing factors of chronic active gastritis and peptic ulcer and is closely associated with the occurrence and progression of gastric cancer. CagA protein is a major virulence factor of H. pylori that interacts with SHP-2, a true oncogene, to interfere with cellular signaling pathways; CagA also plays a crucial role in promoting the carcinogenesis of gastric epithelial cells. However, currently, the molecular mechanisms of gastric epithelial cells that antagonize CagA pathogenesis remain inconclusive. Methods:  We showed that AGS gastric cancer cells transfected with CagA exhibited the inhibition of proliferation and increased activity of caspase 3/7 using two-dimensional gel electrophoresis and secondary mass spectrometry (MS/MS). Results:  It was found that the AGS gastric cancer cells stably expressing CagA displayed significantly increased the expression of 16 proteins, including hnRNPC1/2. Further analysis revealed that hnRNPC1/2 significantly boosted the expression of the p27(kip1) protein. Conclusion: Our data suggested that hnRNPC1/2 upregulates p27(kip1) expression and the subsequent suppression of cell proliferation and induction of apoptosis, thereby providing an important mechanism whereby gastric epithelial cells antagonize CagA-mediated pathogenesis.
Authors:
Shenghang Jin; Minjin Wu; Hanqin Cao; Sunke Ying; Jiping Hua; Yu Chen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Helicobacter     Volume:  17     ISSN:  1523-5378     ISO Abbreviation:  Helicobacter     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9605411     Medline TA:  Helicobacter     Country:  United States    
Other Details:
Languages:  eng     Pagination:  140-7     Citation Subset:  IM    
Copyright Information:
© 2012 Blackwell Publishing Ltd.
Affiliation:
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Department of Laboratory Medicine, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China.
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