Document Detail


p16(INK4A) and Ki-67 immunostaining on cell blocks from residual ThinPrep material is helpful in identifying significant preneoplastic cervical lesions.
MedLine Citation:
PMID:  21440378     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cell blocks can be prepared from residual ThinPrep material, and immunohistochemical staining can be used. The objectives of the current study were (1) to investigate the role of cell block preparation in identifying significant preneoplastic cervical lesions; and (2) to assess the diagnostic value of p16(INK4A) and Ki-67 immunostaining on cell blocks to identify significant preneoplastic cervical lesions. Samples from residual substances of ThinPrep from 79 patients were collected for cell block preparations. Cell block sections and histological sections of the same patients were stained with hematoxylin and eosin, and were immunostained with antibodies against p16(INK4A) and Ki-67 antigen. The sensitivity and specificity for p16(INK4A) and Ki-67 immunostaining to detect high-grade squamous intraepithelial neoplasia were 95.12% and 73.68%, respectively. The positive predictive value and negative predictive value were 79.59% and 93.33%, respectively. Immunostaining of cell blocks for p16(INK4A) and Ki-67 exhibited a statistically significant association with the presence of significant lesions on cell blocks (P<0.05). p16(INK4A) and Ki-67 immunostaining on cell blocks from residual ThinPrep material is helpful in identifying significant preneoplastic cervical lesions.
Authors:
Huiqiong Bao; Yilin Wu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-25
Journal Detail:
Title:  Pathology, research and practice     Volume:  -     ISSN:  1618-0631     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7806109     Medline TA:  Pathol Res Pract     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier GmbH. All rights reserved.
Affiliation:
Department of Gynecology, Guangdong General Hospital & Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, China.
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