Document Detail


p16 inhibition of transformed and primary squamous epithelial cells.
MedLine Citation:
PMID:  8649826     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We and others have recently shown that p16 can potently and specifically inhibit progression through the G1 phase of the replicative cycle in cells that express the retinoblastoma protein (pRB). However, none of these studies examined cell types in which p16 has been firmly implicated in tumorigenesis. We predicted that such cells would show sensitivity to p16 inhibition, perhaps conferred by proteins in addition to or other than pRB. Intragenic, inactivating mutations of p16 have been found at significant frequency in primary tumors derived from squamous epithelial cells of the esophagus (ESCC). We therefore examined p16 function in ESCC lines and in primary squamous epithelial cells cultured from mouse skin. We find that seven of eight ESCC lines tested are inhibited by p16 and fail to express the protein endogenously. The lone p16-resistant line expresses endogenous p16 but lacks functional pRB. Primary squamous epithelial cells are also inhibited by p16. These data suggest that squamous epithelial cells are generally sensitive to inhibition by a regulatory pathway that involves p16 and pRB, and that, by the time of establishment in culture, there is nearly universal inactivation of this pathway in ESCCs.
Authors:
G H Enders; J Koh; C Missero; A K Rustgi; E Harlow
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Oncogene     Volume:  12     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-07-25     Completed Date:  1996-07-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1239-45     Citation Subset:  IM    
Affiliation:
Cancer Center, Massachusetts General Hospital, Boston 02129, USA.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells / pathology,  physiology
Animals
Carcinoma, Squamous Cell / pathology*
Carrier Proteins / genetics,  physiology*
Cell Transformation, Neoplastic / pathology*
Cells, Cultured
Cyclin-Dependent Kinase Inhibitor p16
Epithelial Cells
Epithelium / physiology
Exons
Humans
Keratinocytes / cytology,  physiology*
Mice
Mice, Inbred SENCAR
Microinjections
Retinoblastoma Protein / physiology
Sensitivity and Specificity
Skin / cytology
Skin Neoplasms / pathology*
Skin Physiological Phenomena
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Cyclin-Dependent Kinase Inhibitor p16; 0/Retinoblastoma Protein

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