Document Detail


p12 tethers the murine leukemia virus pre-integration complex to mitotic chromosomes.
MedLine Citation:
PMID:  23300449     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The p12 protein of the murine leukemia virus (MLV) is a constituent of the pre-integration complex (PIC) but its function in this complex remains unknown. We developed an imaging system to monitor MLV PIC trafficking in live cells. This allowed the visualization of PIC docking to mitotic chromosomes and its release upon exit from mitosis. Docking occurred concomitantly with nuclear envelope breakdown and was impaired for PICs of viruses with lethal p12 mutations. Insertion of a heterologous chromatin binding module into p12 of one of these mutants restored PICs attachment to the chromosomes and partially rescued virus replication. Capsid dissociated from wild type PICs in mitotic cells but remained associated with PICs harboring tethering-negative p12 mutants. Altogether, these results explain, in part, MLV restriction to dividing cells and reveal a role for p12 as a factor that tethers MLV PIC to mitotic chromosomes.
Authors:
Efrat Elis; Marcelo Ehrlich; Adi Prizan-Ravid; Nihay Laham-Karam; Eran Bacharach
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-12-27
Journal Detail:
Title:  PLoS pathogens     Volume:  8     ISSN:  1553-7374     ISO Abbreviation:  PLoS Pathog.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2013-01-09     Completed Date:  2013-06-27     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101238921     Medline TA:  PLoS Pathog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e1003103     Citation Subset:  IM    
Affiliation:
Department of Cell Research and Immunology, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Capsid
Capsid Proteins / genetics,  metabolism
Cell Line
Chromatin / metabolism
Chromosomes / virology*
Gene Products, gag / genetics*,  metabolism*
Leukemia Virus, Murine / genetics*
Mice
Mitosis
Mutation
Nuclear Envelope / pathology,  virology
Protein Binding
Virus Attachment
Virus Integration
Virus Replication / genetics
Chemical
Reg. No./Substance:
0/Capsid Proteins; 0/Chromatin; 0/Gag protein p12, moloney murine leukemia virus; 0/Gene Products, gag
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