Document Detail


p-Chlorophenoxyisobutyric acid impairs auxin response in Arabidopsis root.
MedLine Citation:
PMID:  14526108     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
p-Chlorophenoxyisobutyric acid (PCIB) is known as a putative antiauxin and is widely used to inhibit auxin action, although the mechanism of PCIB-mediated inhibition of auxin action is not characterized very well at the molecular level. In the present work, we showed that PCIB inhibited BA::beta-glucuronidase (GUS) expression induced by indole-3-acetic acid (IAA), 2,4-dichlorophenoxyacetic acid, and 1-naphthaleneacetic acid. PCIB also inhibited auxin-dependent DR5::GUS expression. RNA hybridization and quantitative reverse transcriptase-polymerase chain reaction analyses suggested that PCIB reduced auxin-induced accumulation of transcripts of Aux/IAA genes. In addition, PCIB relieved the reduction of GUS activity in HS::AXR3NT-GUS transgenic line in which auxin inhibits GUS activity by promoting degradation of the AXR3NT-GUS fusion protein. Physiological analysis revealed that PCIB inhibited lateral root production, gravitropic response of roots, and growth of primary roots. These results suggest that PCIB impairs auxin-signaling pathway by regulating Aux/IAA protein stability and thereby affects the auxin-regulated Arabidopsis root physiology.
Authors:
Yutaka Oono; Chiharu Ooura; Abidur Rahman; Evalour T Aspuria; Ken-ichiro Hayashi; Atsushi Tanaka; Hirofumi Uchimiya
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-10-02
Journal Detail:
Title:  Plant physiology     Volume:  133     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-11-12     Completed Date:  2004-03-24     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1135-47     Citation Subset:  IM    
Affiliation:
Department of Ion-beam-applied Biology, Japan Atomic Energy Research Institute, Takasaki 370-1292, Japan. yoono@taka.jaeri.go.jp
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MeSH Terms
Descriptor/Qualifier:
2,4-Dichlorophenoxyacetic Acid / pharmacology
Arabidopsis / drug effects,  genetics,  growth & development*
Clofibric Acid / pharmacology*
Gene Expression Regulation, Developmental / drug effects
Gene Expression Regulation, Plant / drug effects
Glucuronidase / drug effects,  metabolism
Indoleacetic Acids / pharmacology*
Naphthaleneacetic Acids / pharmacology
Plant Growth Regulators / pharmacology
Plant Roots / drug effects,  genetics,  growth & development*
Signal Transduction / drug effects,  physiology
Chemical
Reg. No./Substance:
0/Indoleacetic Acids; 0/Naphthaleneacetic Acids; 0/Plant Growth Regulators; 33T7G7757C/1-naphthaleneacetic acid; 6U1S09C61L/indoleacetic acid; 882-09-7/Clofibric Acid; 94-75-7/2,4-Dichlorophenoxyacetic Acid; EC 3.2.1.31/Glucuronidase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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