| p-Aminophenol-induced cytotoxicity in Jurkat T Cells: Protective effect of 2(RS)-n-propylthiazolidine-4(R)-carboxylic acid. | |
| | |
MedLine Citation:
|
PMID: 22162043 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Acetaminophen (APAP) is hepatotoxic and can cause toxicity in Jurkat T cells. p-Aminophenol (PAP), an industrial chemical and APAP metabolite, is nephrotoxic and hepatotoxic. Its potential toxicity in Jurkat T cells was investigated. PAP (10-250 µM) caused toxicity (decreased survival and increased LDH activity in incubation medium) and GSH depletion. At a concentration of 100 µM but not 250 µM, PAP increased DNA fragmentation. It decreased p-Akt levels (Elisa) and at higher concentrations decreased p-Akt expression (Western blotting). It had no effect on FasL expression. The cysteine precursor 2(RS)-n-propylthiazolidine-4(R)-carboxylic acid (250 µM) attenuated the PAP (100 µM)-induced decrease in viability and prevented GSH depletion and increased DNA fragmentation. It attenuated the PAP-induced decrease in p-Akt levels and protected against the decrease in p-Akt expression. The results demonstrate PAP-induced toxicity and suggest that it is due at least in part to apoptosis and involves GSH depletion and p-Akt inactivation. © 2011 Wiley Periodicals, Inc. J Biochem Mol Toxicol 00:1-8 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20402. |
| | |
Authors:
|
Weiyuan Chang; Shrish Barve; Theresa S Chen |
Related Documents
:
|
11042363 - Protein kinase c iota protects neural cells against apoptosis induced by amyloid beta-p... 12428763 - Acute neuronal injury, excitotoxicity, and the endocannabinoid system. 11371023 - Human alveolar macrophages express elafin and secretory leukocyte protease inhibitor. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-12-8 |
Journal Detail:
|
Title: Journal of biochemical and molecular toxicology Volume: - ISSN: 1099-0461 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2011-12-13 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9717231 Medline TA: J Biochem Mol Toxicol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 Wiley Periodicals, Inc. |
Affiliation:
|
Department of Pharmacology and Toxicology, University of Louisville, Louisville, KY 40292, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Adenoviral load diagnostics by quantitative polymerase chain reaction: techniques and application.
Next Document: Sonoluminescence of Uranyl Ions in Aqueous Solutions.