Document Detail

Oxalate efflux transporter from the brown rot fungus Fomitopsis palustris.
MedLine Citation:
PMID:  20889782     Owner:  NLM     Status:  MEDLINE    
An oxalate-fermenting brown rot fungus, Fomitopsis palustris, secretes large amounts of oxalic acid during wood decay. Secretion of oxalic acid is indispensable for the degradation of wood cell walls, but almost nothing is known about the transport mechanism by which oxalic acid is secreted from F. palustris hyphal cells. We characterized the mechanism for oxalate transport using membrane vesicles of F. palustris. Oxalate transport in F. palustris was ATP dependent and was strongly inhibited by several inhibitors, such as valinomycin and NH(4)(+), suggesting the presence of a secondary oxalate transporter in this fungus. We then isolated a cDNA, FpOAR (Fomitopsis palustris oxalic acid resistance), from F. palustris by functional screening of yeast transformants with cDNAs grown on oxalic acid-containing plates. FpOAR is predicted to be a membrane protein that possesses six transmembrane domains but shows no similarity with known oxalate transporters. The yeast transformant possessing FpOAR (FpOAR-transformant) acquired resistance to oxalic acid and contained less oxalate than the control transformant. Biochemical analyses using membrane vesicles of the FpOAR-transformant showed that the oxalate transport property of FpOAR was consistent with that observed in membrane vesicles of F. palustris. The quantity of FpOAR transcripts was correlated with increasing oxalic acid accumulation in the culture medium and was induced when exogenous oxalate was added to the medium. These results strongly suggest that FpOAR plays an important role in wood decay by acting as a secondary transporter responsible for secretion of oxalate by F. palustris.
Tomoki Watanabe; Nobukazu Shitan; Shiro Suzuki; Toshiaki Umezawa; Mikio Shimada; Kazufumi Yazaki; Takefumi Hattori
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-01
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  76     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-22     Completed Date:  2011-02-25     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7683-90     Citation Subset:  IM    
Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan.
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MeSH Terms
Adenosine Triphosphate / metabolism
Cluster Analysis
Coriolaceae / enzymology*,  genetics,  metabolism*
DNA, Complementary / genetics,  isolation & purification
DNA, Fungal / chemistry,  genetics
Enzyme Inhibitors / metabolism
Gene Expression Profiling
Membrane Transport Proteins / genetics*,  metabolism*
Molecular Sequence Data
Oxalates / metabolism*
Quaternary Ammonium Compounds / metabolism
Secretory Vesicles / enzymology
Sequence Analysis, DNA
Sequence Homology
Valinomycin / metabolism
Wood / metabolism,  microbiology
Reg. No./Substance:
0/DNA, Complementary; 0/DNA, Fungal; 0/Enzyme Inhibitors; 0/Membrane Transport Proteins; 0/Oxalates; 0/Quaternary Ammonium Compounds; 2001-95-8/Valinomycin; 56-65-5/Adenosine Triphosphate

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