| Oxalate efflux transporter from the brown rot fungus Fomitopsis palustris. | |
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MedLine Citation:
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PMID: 20889782 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An oxalate-fermenting brown rot fungus, Fomitopsis palustris, secretes large amounts of oxalic acid during wood decay. Secretion of oxalic acid is indispensable for the degradation of wood cell walls, but almost nothing is known about the transport mechanism by which oxalic acid is secreted from F. palustris hyphal cells. We characterized the mechanism for oxalate transport using membrane vesicles of F. palustris. Oxalate transport in F. palustris was ATP dependent and was strongly inhibited by several inhibitors, such as valinomycin and NH(4)(+), suggesting the presence of a secondary oxalate transporter in this fungus. We then isolated a cDNA, FpOAR (Fomitopsis palustris oxalic acid resistance), from F. palustris by functional screening of yeast transformants with cDNAs grown on oxalic acid-containing plates. FpOAR is predicted to be a membrane protein that possesses six transmembrane domains but shows no similarity with known oxalate transporters. The yeast transformant possessing FpOAR (FpOAR-transformant) acquired resistance to oxalic acid and contained less oxalate than the control transformant. Biochemical analyses using membrane vesicles of the FpOAR-transformant showed that the oxalate transport property of FpOAR was consistent with that observed in membrane vesicles of F. palustris. The quantity of FpOAR transcripts was correlated with increasing oxalic acid accumulation in the culture medium and was induced when exogenous oxalate was added to the medium. These results strongly suggest that FpOAR plays an important role in wood decay by acting as a secondary transporter responsible for secretion of oxalate by F. palustris. |
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Authors:
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Tomoki Watanabe; Nobukazu Shitan; Shiro Suzuki; Toshiaki Umezawa; Mikio Shimada; Kazufumi Yazaki; Takefumi Hattori |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-01 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 76 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-22 Completed Date: 2011-02-25 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 7683-90 Citation Subset: IM |
Affiliation:
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Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AB372882 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Cluster Analysis Coriolaceae / enzymology*, genetics, metabolism* DNA, Complementary / genetics, isolation & purification DNA, Fungal / chemistry, genetics Enzyme Inhibitors / metabolism Gene Expression Profiling Membrane Transport Proteins / genetics*, metabolism* Molecular Sequence Data Oxalates / metabolism* Phylogeny Quaternary Ammonium Compounds / metabolism Secretory Vesicles / enzymology Sequence Analysis, DNA Sequence Homology Valinomycin / metabolism Wood / metabolism, microbiology |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/DNA, Fungal; 0/Enzyme Inhibitors; 0/Membrane Transport Proteins; 0/Oxalates; 0/Quaternary Ammonium Compounds; 2001-95-8/Valinomycin; 56-65-5/Adenosine Triphosphate |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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