Document Detail

An overview of botanical clearing technique.
MedLine Citation:
PMID:  49949     Owner:  NLM     Status:  MEDLINE    
Clearing techniques are outlined with reference to their action on the chemical constituents of plant tissue. The most general technique would include pretreatment with solvents, dissolution of protoplasm, dissolution of other substances, bleaching, infiltration with a dense fluid, and staining. Extensive chemical changes go on during these steps and may prevent satisfactory clearing, an important example being the discoloration of phenolic compounds. Rational design of clearing methods for the chemically distinct cell types and tissue seems a likely future development.
R O Gardner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Stain technology     Volume:  50     ISSN:  0038-9153     ISO Abbreviation:  Stain Technol     Publication Date:  1975 Mar 
Date Detail:
Created Date:  1975-09-13     Completed Date:  1975-09-13     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0404535     Medline TA:  Stain Technol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  99-105     Citation Subset:  IM    
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MeSH Terms
Histological Techniques / methods*
Hydrochloric Acid
Phenols / metabolism
Pigments, Biological / metabolism
Staining and Labeling
Reg. No./Substance:
0/Alkalies; 0/Phenols; 0/Pigments, Biological; 7647-01-0/Hydrochloric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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