Document Detail

p55Cdc/cdc20 overexpression promotes early g1/s transition in myeloid cells.
MedLine Citation:
PMID:  11359945     Owner:  NLM     Status:  MEDLINE    
p55Cdc/Cdc20 is expressed in cycling mammalian cells and has been shown to be an activator of the mitotic spindle assembly checkpoint. We previously showed that overexpression of p55Cdc/Cdc20 in myeloid cells resulted in accelerated apoptosis and inhibition of granulocyte differentiation in the murine myeloid cell line 32Dcl3. p55Cdc/Cdc20 protein expression is detected in cells at late G1 phase of the cell cycle but is maximal during G2 phase. We report in this paper that inducible expression of p55Cdc/Cdc20 in 32Dcl3 cells results in premature transition from G1 to S phase. To characterize the mechanism of this early transition, we examined the expression of critical regulatory proteins during the cell cycle. Although expression of cyclin D, cyclin E, cdk2, and cdc2 did not change significantly between p55Cdc/Cdc20-overexpressing and control cells, p27Kip1 protein levels were lower and cdk2 activity higher during G1 to S transition in p55Cdc/Cdc20-overexpressing cells compared to control cells. Cyclin B1 levels were lower at early G1 phase in cells overexpressing p55Cdc/Cdc20. Our results suggest that p55Cdc/Cdc20 may play an important role in G1 to S transition during myelopoiesis.
M L Lin; K M Sakamoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Stem cells (Dayton, Ohio)     Volume:  19     ISSN:  1066-5099     ISO Abbreviation:  Stem Cells     Publication Date:  2001  
Date Detail:
Created Date:  2001-05-21     Completed Date:  2001-07-26     Revised Date:  2012-06-25    
Medline Journal Info:
Nlm Unique ID:  9304532     Medline TA:  Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  205-11     Citation Subset:  IM    
Department of Pediatrics, UCLA School of Medicine, Los Angeles, California 90095-1752, USA.
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MeSH Terms
Blotting, Northern
Blotting, Western
CDC2 Protein Kinase / biosynthesis
CDC2-CDC28 Kinases*
Cell Cycle
Cell Cycle Proteins*
Cell Differentiation
Cell Line
Cyclin D
Cyclin E / biosynthesis
Cyclin-Dependent Kinase 2
Cyclin-Dependent Kinase Inhibitor p27
Cyclin-Dependent Kinases / biosynthesis
Cyclins / biosynthesis
G1 Phase*
Granulocytes / metabolism
Microtubule-Associated Proteins / biosynthesis
Myeloid Cells / metabolism*
Protein Biosynthesis*
Protein-Serine-Threonine Kinases / biosynthesis
Proteins / physiology*
RNA / metabolism
S Phase*
Time Factors
Tumor Suppressor Proteins*
Grant Support
Reg. No./Substance:
0/Cdc20 protein, mouse; 0/Cdkn1b protein, mouse; 0/Cell Cycle Proteins; 0/Cyclin D; 0/Cyclin E; 0/Cyclins; 0/Microtubule-Associated Proteins; 0/Proteins; 0/Tumor Suppressor Proteins; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; 156288-95-8/CDC20 protein, human; 63231-63-0/RNA; EC Kinases; EC Protein Kinase; EC Kinases; EC protein, human; EC protein, mouse; EC Kinase 2; EC Kinases

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