Document Detail

The ovarian cancer-derived secretory/releasing proteome: a repertoire of tumor markers.
MedLine Citation:
PMID:  22623176     Owner:  NLM     Status:  Publisher    
Ovarian cancer is the most lethal gynecological malignancy worldwide, and early detection of this disease using serum or plasma biomarkers may improve its clinical outcome. In the present study, a large scale protein database derived from ovarian cancer was created to enable tumor marker discovery. First, primary organ cultures were established with the tumor tissues and corresponding normal tissues obtained from six ovarian cancer patients, and the serum-free conditioned medium (CM) samples were collected for proteomic analysis. The total proteins from the CM sample were separated by SDS-PAGE, digested with trypsin and then analyzed by LC-MS/MS. Combining data from the tumor tissues and the normal tissues, 1129 proteins were identified in total, of which those categorized as 'extracellular proteins' and 'plasma membrane proteins' accounted for 21.4% and 16.9%, respectively. For validation, three secretory proteins (NID1, TIMP2 and VCAN) that involved in 'organ development'-associated sub-network, showed significant differences between their levels in the circulating plasma samples from ovarian cancer patients and healthy women. In conclusion, this ovarian cancer-derived protein database provides a credible repertoire of potential biomarkers in blood for this malignant disease, and deserves mining further.
Ying Zhang; Bin Xu; Yu Liu; Hongwen Yao; Nenan Lu; Bin Li; Jia Gao; Suping Guo; Naijun Han; Jun Qi; Kaitai Zhang; Shujun Cheng; Hongxia Wang; Xuemin Zhang; Ting Xiao; Lingying Wu; Yanning Gao
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-23
Journal Detail:
Title:  Proteomics     Volume:  -     ISSN:  1615-9861     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
State Key Laboratory of Molecular Oncology, Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Cancer Institute (Hospital), Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, 100021, China.
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