Document Detail

The osmotic pressure of chondroitin sulphate solutions: experimental measurements and theoretical analysis.
MedLine Citation:
PMID:  10656048     Owner:  NLM     Status:  MEDLINE    
We used equilibrium dialysis to measure the osmotic pressure of chondroitin sulphate (CS) solutions as a function of their concentration and fixed charge density (FCD) and the ionic strength and composition of the solution. Osmotic pressure varied nonlinearly with the concentration of chondroitin sulphate and in 0.15 M NaCl at FCDs typical of uncompressed cartilage (approximately 0.4 mmol/g extrafibrillar H2O) was approximately 3 atmospheres. Osmotic pressure fell by 60% as solution ionic strength increased up to about 1 M, but remained relatively constant at higher ionic strengths. The ratio of Ca2+ to Na+ in the medium was a minor determinant of osmotic pressure. The data are compared with a theoretical model of the electrostatic contribution to osmotic pressure calculated from the Poisson-Boltzmann equation using a rod-in-cell model for CS. The effective radius of the polyelectrolyte rod is taken as a free parameter. The model qualitatively reproduces the non-linear concentration dependence, but underestimates the osmotic pressure by an amount that is independent of ionic strength. This difference, presumably arising from oncotic and entropic effects, is approximately 1/3 of the total osmotic pressure at physiological polymer concentrations and ionic strength.
S Ehrlich; N Wolff; R Schneiderman; A Maroudas; K H Parker; C P Winlove
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biorheology     Volume:  35     ISSN:  0006-355X     ISO Abbreviation:  Biorheology     Publication Date:    1998 Nov-Dec
Date Detail:
Created Date:  2000-02-14     Completed Date:  2000-02-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372526     Medline TA:  Biorheology     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  383-97     Citation Subset:  IM    
Department of Biomedical Engineering, Technion, Haifa, Israel.
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MeSH Terms
Cartilage / physiology*
Chondroitin Sulfates / physiology*
Models, Biological
Osmolar Concentration
Osmotic Pressure
Reg. No./Substance:
7440-23-5/Sodium; 7440-70-2/Calcium; 9007-28-7/Chondroitin Sulfates

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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