| The Bax/Bak ortholog in Drosophila, Debcl, exerts limited control over programmed cell death. | |
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MedLine Citation:
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PMID: 19088092 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bcl-2 family members are pivotal regulators of programmed cell death (PCD). In mammals, pro-apoptotic Bcl-2 family members initiate early apoptotic signals by causing the release of cytochrome c from the mitochondria, a step necessary for the initiation of the caspase cascade. Worms and flies do not show a requirement for cytochrome c during apoptosis, but both model systems express pro- and anti-apoptotic Bcl-2 family members. Drosophila encodes two Bcl-2 family members, Debcl (pro-apoptotic) and Buffy (anti-apoptotic). To understand the role of Debcl in Drosophila apoptosis, we produced authentic null alleles at this locus. Although gross development and lifespans were unaffected, we found that Debcl was required for pruning cells in the developing central nervous system. debcl genetically interacted with the ced-4/Apaf1 counterpart dark, but was not required for killing by RHG (Reaper, Hid, Grim) proteins. We found that debcl(KO) mutants were unaffected for mitochondrial density or volume but, surprisingly, in a model of caspase-independent cell death, heterologous killing by murine Bax required debcl to exert its pro-apoptotic activity. Therefore, although debcl functions as a limited effector of PCD during normal Drosophila development, it can be effectively recruited for killing by mammalian members of the Bcl-2 gene family. |
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Authors:
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Kathleen A Galindo; Wan-Jin Lu; Jae H Park; John M Abrams |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-12-15 |
Journal Detail:
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Title: Development (Cambridge, England) Volume: 136 ISSN: 0950-1991 ISO Abbreviation: Development Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2008-12-23 Completed Date: 2009-03-18 Revised Date: 2012-10-09 |
Medline Journal Info:
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Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
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Languages: eng Pagination: 275-83 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9039, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified Apoptosis / genetics, physiology* Autophagy / genetics, physiology Base Sequence Central Nervous System / cytology, embryology DNA Primers / genetics Drosophila / cytology*, embryology, genetics, physiology* Drosophila Proteins / genetics, physiology* Female Gene Targeting Genes, Insect Male Membrane Proteins / genetics, physiology* Mice Mutation Species Specificity bcl-2-Associated X Protein / genetics, physiology |
| Grant Support | |
ID/Acronym/Agency:
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F31GM68987/GM/NIGMS NIH HHS; GM072124/GM/NIGMS NIH HHS; R56 GM072124/GM/NIGMS NIH HHS; R56 GM072124-14/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bax protein, mouse; 0/DNA Primers; 0/Drosophila Proteins; 0/Membrane Proteins; 0/bcl-2-Associated X Protein; 0/debcl protein, Drosophila |
| Comments/Corrections | |
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