Document Detail


The orphan nuclear receptor SHP inhibits apoptosis during the monocytic differentiation by inducing p21WAF1.
MedLine Citation:
PMID:  19322021     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Small heterodimer partner (SHP) is an atypical member of nuclear receptor superfamily that lacks a DNA-binding domain. In previous study, we showed that SHP, c-jun, p65 of NF-gammaB subunits, and p21WAF1 expression was increased during monocytic differentiaton with the exposure of human leukemia cells to a differentiation agent, PMA. In this study, c-Jun and p65 were shown to mediate the transcriptional activation of the SHP promoter. In addition, SHP induced the cell cycle regulatory protein levels and cooperatively increased an induction of p21WAF1 expression with p65. Furthermore, SHP protected differentiated cells from etoposide-induced cellular apoptosis through the induction and cytoplasmic sequestration of p21WAF1. Complex formation between SHP and p21WAF1 was demonstrated by means of coimmunoprecipitation. These results suggest that SHP prolongs a cellular survival of differentiating monocytes through the transcriptional regulation of target genes of cell survival and differentiation.
Authors:
Kyeongjin Kim; Yoon Ha Choi; Hyeong Hoe Kim; Jaehun Cheong
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental & molecular medicine     Volume:  41     ISSN:  1226-3613     ISO Abbreviation:  Exp. Mol. Med.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-29     Completed Date:  2009-07-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9607880     Medline TA:  Exp Mol Med     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  429-39     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology, Pusan National University, Busan 609-735, Korea.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis*
Cell Differentiation
Cell Line, Tumor
Cyclin-Dependent Kinase Inhibitor p21 / genetics,  metabolism*
Gene Expression Regulation
Humans
Monocytes / cytology
Promoter Regions, Genetic
Proto-Oncogene Proteins c-jun / genetics,  metabolism
Receptors, Cytoplasmic and Nuclear / genetics,  metabolism*
Transcription Factor RelA / genetics,  metabolism
Chemical
Reg. No./Substance:
0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Proto-Oncogene Proteins c-jun; 0/Receptors, Cytoplasmic and Nuclear; 0/Transcription Factor RelA; 0/nuclear receptor subfamily 0, group B, member 2
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