Document Detail


An origin unwinding activity regulates initiation of DNA replication during mammalian cell cycle.
MedLine Citation:
PMID:  2843984     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An in vitro assay was developed to study the positive factors that regulate the onset of DNA replication during the mammalian cell cycle. Extracts prepared from cells at defined positions in the cell cycle were used to examine the replication of SV40 DNA in a cell free system. Extracts prepared from S phase cells were ten times more efficient at initiating replication at the SV40 origin than were extracts from G1 cells, whereas elongation rates were similar in G1 and S reactions. At a discrete point in the cell cycle, just before the cell's entry into S, an activity appeared that was required, in conjunction with SV40 T antigen, for site specific initiation at the SV40 origin. This factor had a role in unwinding DNA at the replication origin.
Authors:
J M Roberts; G D'Urso
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  241     ISSN:  0036-8075     ISO Abbreviation:  Science     Publication Date:  1988 Sep 
Date Detail:
Created Date:  1988-10-25     Completed Date:  1988-10-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1486-9     Citation Subset:  IM    
Affiliation:
Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Polyomavirus Transforming / physiology
Cell Cycle*
Cell Line
Cell-Free System
DNA Replication*
Humans
Interphase
Simian virus 40 / genetics
Virus Replication
Grant Support
ID/Acronym/Agency:
AG0005/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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