Document Detail

The origin recognition complex has essential functions in transcriptional silencing and chromosomal replication.
MedLine Citation:
PMID:  7774809     Owner:  NLM     Status:  MEDLINE    
The role of the origin recognition complex (ORC) was investigated in replication initiation and in silencing. Temperature-sensitive mutations in ORC genes caused defects in replication initiation at chromosomal origins of replication, as measured by two-dimensional (2-D) origin-mapping gels, fork migration analysis, and plasmid replication studies. These data were consistent with ORC functioning as a eukaryotic replication initiator. Some origins displayed greater replication initiation deficiencies in orc mutants than did others, revealing functional differences between origins. Alleles of ORC5 were isolated that were defective for silencing but not replication, indicating that ORC's role in silencing could be separated from its role in replication. In temperature-sensitive orc mutants arrested in mitosis, temperature-shift experiments caused a loss of silencing, indicating both that ORC had functions outside of the S phase of the cell cycle and that ORC was required for the maintenance of the silenced state.
C A Fox; S Loo; A Dillin; J Rine
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genes & development     Volume:  9     ISSN:  0890-9369     ISO Abbreviation:  Genes Dev.     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-07-07     Completed Date:  1995-07-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8711660     Medline TA:  Genes Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  911-24     Citation Subset:  IM    
Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
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MeSH Terms
Cell Cycle / genetics
Chromosomes, Fungal / genetics*
DNA Replication*
DNA, Fungal / genetics
DNA-Binding Proteins / metabolism*
Eukaryotic Cells
Fungal Proteins / genetics
Genes, Fungal / genetics
Nucleic Acid Conformation
Origin Recognition Complex
Plasmids / genetics
Replication Origin*
Repressor Proteins / genetics
Saccharomyces cerevisiae Proteins
Transcription, Genetic*
Yeasts / genetics*
Grant Support
Reg. No./Substance:
0/DNA, Fungal; 0/DNA-Binding Proteins; 0/Fungal Proteins; 0/ORC5 protein, S cerevisiae; 0/Origin Recognition Complex; 0/Repressor Proteins; 0/Saccharomyces cerevisiae Proteins

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