Document Detail

The origin of cells that repopulate patellar tendons used for reconstructing anterior cruciate ligaments in man.
MedLine Citation:
PMID:  12892205     Owner:  NLM     Status:  MEDLINE    
Cryopreserved patellar tendon allografts are often recommended for reconstruction of anterior cruciate ligaments (ACLs) because living donor fibroblasts are thought to promote repair. Animal studies, however, indicate that ligaments regenerate from recipient rather than donor cells. If applicable to man, these observations suggest that allograft cell viability is unimportant. We therefore used short tandem repeat analysis with polymerase chain reaction (PCR) amplification to determine the source of cells in nine human ACLs reconstructed with cryopreserved patellar tendon allografts. PCR amplification of donor and recipient DNA obtained before operation and DNA from the graft obtained two to ten months after transplantation revealed the genotype of cells and showed only recipient cells in the graft area. Rather than preserve the viability of donor cells, a technique is required which will facilitate the introduction of recipient cells into patellar tendon allografts.
B H Min; M S Han; J I Woo; H J Park; S R Park
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of bone and joint surgery. British volume     Volume:  85     ISSN:  0301-620X     ISO Abbreviation:  J Bone Joint Surg Br     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-08-01     Completed Date:  2003-08-28     Revised Date:  2010-11-10    
Medline Journal Info:
Nlm Unique ID:  0375355     Medline TA:  J Bone Joint Surg Br     Country:  England    
Other Details:
Languages:  eng     Pagination:  753-7     Citation Subset:  AIM; IM    
Inha University College of Medicine, Incheon, Korea.
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MeSH Terms
Anterior Cruciate Ligament / cytology*
Cryopreservation / methods
DNA / analysis
Polymerase Chain Reaction / methods
Tandem Repeat Sequences / genetics
Tendons / cytology*,  transplantation
Transplantation, Homologous
Reg. No./Substance:

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