| The origin of cells that repopulate patellar tendons used for reconstructing anterior cruciate ligaments in man. | |
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MedLine Citation:
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PMID: 12892205 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cryopreserved patellar tendon allografts are often recommended for reconstruction of anterior cruciate ligaments (ACLs) because living donor fibroblasts are thought to promote repair. Animal studies, however, indicate that ligaments regenerate from recipient rather than donor cells. If applicable to man, these observations suggest that allograft cell viability is unimportant. We therefore used short tandem repeat analysis with polymerase chain reaction (PCR) amplification to determine the source of cells in nine human ACLs reconstructed with cryopreserved patellar tendon allografts. PCR amplification of donor and recipient DNA obtained before operation and DNA from the graft obtained two to ten months after transplantation revealed the genotype of cells and showed only recipient cells in the graft area. Rather than preserve the viability of donor cells, a technique is required which will facilitate the introduction of recipient cells into patellar tendon allografts. |
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Authors:
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B H Min; M S Han; J I Woo; H J Park; S R Park |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of bone and joint surgery. British volume Volume: 85 ISSN: 0301-620X ISO Abbreviation: J Bone Joint Surg Br Publication Date: 2003 Jul |
Date Detail:
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Created Date: 2003-08-01 Completed Date: 2003-08-28 Revised Date: 2010-11-10 |
Medline Journal Info:
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Nlm Unique ID: 0375355 Medline TA: J Bone Joint Surg Br Country: England |
Other Details:
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Languages: eng Pagination: 753-7 Citation Subset: AIM; IM |
Affiliation:
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Inha University College of Medicine, Incheon, Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Anterior Cruciate Ligament / cytology* Cryopreservation / methods DNA / analysis Female Genotype Humans Male Patella* Polymerase Chain Reaction / methods Tandem Repeat Sequences / genetics Tendons / cytology*, transplantation Transplantation, Homologous |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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