Document Detail

The origin of catecholaminergic nerve fibers in the subdiaphragmatic vagus nerve of rat.
MedLine Citation:
PMID:  10412834     Owner:  NLM     Status:  MEDLINE    
It is known that the vagus nerve contains catecholaminergic fibers. However, the origin of these fibers has not been systematically examined. In this study, we addressed this issue using retrograde tracing from the subdiaphragmatic vagus nerve combined with immunocytochemistry. The cervical and thoracic sympathetic trunk ganglia, the nodose ganglia and the dorsal motor nucleus of the vagus nerve were examined following injection of Fluoro-Gold or cholera toxin horseradish peroxidase conjugate into the trunks of the subdiaphragmatic vagus nerve of rats. Numerous retrogradely labeled neurons were seen in the nodose ganglion and the dorsal motor nucleus of the vagus nerve. Very few labeled neurons were found in the sympathetic ganglia (less than 0.06% of the neurons in either superior cervical ganglion or cervicothoracic ganglion were retrogradely labeled). Double labeling with immunofluoresence for catecholamine synthesizing enzymes revealed that: (1) 92% of all Fluoro-Gold retrogradely labeled tyrosine hydroxylase immunoreactive neurons were found in parasympathetic sources (75% in the dorsal motor nucleus of the vagus nerve and 17% in the nodose ganglia), and only 8% in the cervicothoracic sympathetic ganglia; (2) 12% of the retrogradely labeled catecholaminergic neurons in the dorsal motor nucleus of the vagus nerve were also dopamine-beta-hydroxylase immunopositive neurons; (3) 70% of the retrogradely labeled neurons in the sympathetic ganglia were tyrosine hydroxylase immunopositive and 54% of these catecholaminergic neurons contained dopamine-beta-hydroxylase, while 30% of the retrogradely labeled neurons were non-catecholaminergic neurons. These results indicate that catecholaminergic fibers in the abdominal vagus nerve are primarily dopaminergic and of parasympathetic origin, and that only an extremely small number of these fibers, mostly noradrenergic in nature, arise from postganglionic sympathetic neurons.
M Yang; X Zhao; R R Miselis
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of the autonomic nervous system     Volume:  76     ISSN:  0165-1838     ISO Abbreviation:  J. Auton. Nerv. Syst.     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-09-01     Completed Date:  1999-09-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8003419     Medline TA:  J Auton Nerv Syst     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  108-17     Citation Subset:  IM    
Department of Animal Biology and the Institute of Neurological Sciences, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104-6045, USA.
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MeSH Terms
Autonomic Nervous System / cytology,  enzymology,  physiology*
Catecholamines / physiology*
Cell Count
Cholera Toxin
Diaphragm / innervation
Fluorescent Dyes
Ganglia, Parasympathetic / cytology,  enzymology,  physiology
Ganglia, Sympathetic / cytology,  enzymology,  physiology
Horseradish Peroxidase
Nerve Fibers / enzymology,  physiology*
Parasympathetic Nervous System / cytology,  enzymology,  physiology
Rats, Sprague-Dawley
Sympathetic Nervous System / cytology,  enzymology,  physiology
Vagus Nerve / enzymology,  physiology*
Grant Support
Reg. No./Substance:
0/2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; 0/Catecholamines; 0/Fluorescent Dyes; 0/Stilbamidines; 0/cholera toxin, B subunit-horseradish peroxidase; 9012-63-9/Cholera Toxin; EC 1.11.1.-/Horseradish Peroxidase

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