| On the origin of the FcRIII (CD16)-containing vesicle population in human neutrophil granulocytes. | |
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MedLine Citation:
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PMID: 1679012 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human blood neutrophils bear two types of Fc receptors that recognize the Fc portion of immunoglobulin G: FcRII and FcRIII. In earlier studies we found that neutrophils not only express FcRIII on their plasma membrane but also contain a large population of FcRIII-containing vesicles mainly located in the juxtanuclear area. To find out whether these vesicles derive from the plasma membrane, we used electron microscopic techniques to study compartments involved in ligand-independent endocytosis in human neutrophil granulocytes. The endocytic compartments were labeled with BSA-gold. This marker entered the cell through non-coated invaginations of the plasma membrane as well as via coated pits. After internalization, BSA-gold was present in numerous electron-lucent vesicles in the juxtanuclear area and in the trans-Golgi reticulum, endosomes, and lysosome-like structures. FcRIII also occurred in the BSA-gold-containing electron-lucent vesicles in the juxtanuclear area, as shown by postembedding immunocytochemical labeling of FcRIII in cells already containing BSA-gold. Quantification showed that 29% of all FcRIII-containing vesicles also bear BSA-gold while the remaining 71% contain only the receptor. In sum, our findings show that one third of the FcRIII-containing electron-lucent vesicles in neutrophil granulocytes derive from the plasma membrane and are involved in ligand-independent endocytosis of FcRIII. The majority of these vesicles, however, are not of an endocytic origin and might constitute an "internal pool" of receptors in these cells. |
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Authors:
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C R Jost; R de Goede; J A Fransen; M R Daha; L A Ginsel |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of cell biology Volume: 54 ISSN: 0171-9335 ISO Abbreviation: Eur. J. Cell Biol. Publication Date: 1991 Apr |
Date Detail:
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Created Date: 1991-10-03 Completed Date: 1991-10-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7906240 Medline TA: Eur J Cell Biol Country: GERMANY |
Other Details:
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Languages: eng Pagination: 313-21 Citation Subset: IM |
Affiliation:
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Laboratory for Electron Microscopy, University of Leiden, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Monoclonal Antigens, CD / metabolism* Antigens, Differentiation / metabolism* Endocytosis* Endosomes / immunology*, ultrastructure Golgi Apparatus / immunology, ultrastructure Humans Lysosomes / immunology, ultrastructure Microscopy, Immunoelectron Neutrophils / immunology*, ultrastructure Receptors, Fc / metabolism* Receptors, IgG Vacuoles / immunology, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigens, CD; 0/Antigens, Differentiation; 0/Receptors, Fc; 0/Receptors, IgG |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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