Document Detail


oriX: a new replication origin in E. coli.
MedLine Citation:
PMID:  6319009     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Replication of the chromosome of E. coli at 42 degrees C in an integratively suppressed dnaA mutant (dnaA46 Sin Hfr) occurs predominantly from the origin of replication of the integrated plasmid (oriV). We have carried out a detailed marker frequency analysis on such Hfrs. This analysis indicates that replication at 42 degrees C occurs not only from oriV, but also from an origin, oriX, located in the terminal region of the chromosome close to, but distinct from, the prophage rac (oriJ). In an oxa1 mutant of one of these Hfrs, we have shown that replication proceeds at 42 degrees C from all three origins: oriV, oriX, and oriC. Loss of the integrated plasmid results in a temperature- and rich-medium-sensitive strain that replicates the chromosome from oriC and oriX. Replication from oriX proceeds slowly and bidirectionally. We suggest that oriX may be involved in the coupling between replication and cell division.
Authors:
B de Massy; J Patte; J M Louarn; J P Bouché
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell     Volume:  36     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1984 Jan 
Date Detail:
Created Date:  1984-03-02     Completed Date:  1984-03-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  221-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics
Chromosomes, Bacterial / metabolism*
DNA Replication*
Escherichia coli / genetics*,  metabolism
Genes, Bacterial*
Mutation
Plasmids
Temperature
Chemical
Reg. No./Substance:
0/Bacterial Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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