Document Detail


The oral microbiota: general overview, taxonomy, and nucleic acid techniques.
MedLine Citation:
PMID:  20717778     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Application of nucleic acid technology to the analysis of the bacterial diversity in the oral cavity in conditions of health and disease has not only confirmed the findings from early culture studies but also significantly expanded the list of oral inhabitants and candidate pathogens associated with the major oral diseases. Over 800 bacterial distinct species-level taxa have been detected in the oral cavity and recent studies using high-throughput technology suggest that the breadth of bacterial diversity can be much larger. This chapter provides an overview of the diversity and taxonomy of oral bacteria. Emphasis is also given on nucleic acid technologies that have been widely used for the study of the oral microbiota.
Authors:
José F Siqueira; Isabela N Rôças
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  666     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-08-18     Completed Date:  2010-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  55-69     Citation Subset:  IM    
Affiliation:
Molecular Microbiology Laboratory, Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, RJ, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Bacteria / classification,  genetics*
Humans
Metagenome / genetics,  physiology*
Metagenomics
Mouth / microbiology*
Mouth Diseases / microbiology
Nucleic Acid Hybridization
Polymerase Chain Reaction

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