Document Detail


An optimized strategy for choosing the number of platelet concentrates to pool.
MedLine Citation:
PMID:  10506446     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CONTEXT: The number of platelet concentrates (PCs) pooled per dose varies widely. The number should be based on quality control statistics. OBJECTIVE: To determine the optimal number of PCs per pool that will achieve a target dose. DESIGN: The population statistics of PC cell counts were obtained from routine quality control records. A target value of 3.00x10(11) cells was chosen. A theoretical model to predict the probability of achieving this target cell count was developed. MAIN OUTCOME MEASURES: The calculated probability was tested in a Monte Carlo simulation. RESULTS: The mean of a sequential series of 100 PC counts (obtained from routine quality control records) was 9.08x10(10), and the standard deviation was 2.70x10(10). Platelet loss during pooling was measured at 8.3%, so that the pooling of 3.27x10(11) cells is required to deliver a dose of 3.00x10(11) cells. The pooling of 4 PCs from this data set predicts an average pool cell count of 3.63+/-2.30x10(11). Using standard tables for normal distribution, the probability of achieving a minimal pool dose of 3.00x10(11) cells is estimated to be greater than 0.76. Similarly, the probability of achieving the target value by pooling 5 PCs is greater than 0.99. In a Monte Carlo simulation selecting 4 PCs, 76.5% of the trials were successful, and for 5 PCs, 99.5% were successful (estimated P values of .77 and .99, respectively). CONCLUSION: Five PCs per pool are necessary and sufficient to achieve the chosen target value given the cell count characteristics of our PC supply.
Authors:
G A Hoeltge; A Shah; J P Miller
Related Documents :
16446996 - Serum protein profile and blood cell counts in adult toads bufo arenarum(amphibia: anur...
18242576 - Effect of topical mitomycin c on corneal endothelium.
10390876 - Rapid isolation of single microbial cells from mixed natural and laboratory populations...
12938186 - An image analysis-based approach for automated counting of cancer cell nuclei in tissue...
11196196 - Highly elevated ultraviolet-induced mutation frequency in isolated chinese hamster cell...
18956326 - The embryonic cell lineage of the nematode rhabditophanes sp.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives of pathology & laboratory medicine     Volume:  123     ISSN:  0003-9985     ISO Abbreviation:  Arch. Pathol. Lab. Med.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-11-03     Completed Date:  1999-11-03     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7607091     Medline TA:  Arch Pathol Lab Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  928-30     Citation Subset:  AIM; IM    
Affiliation:
Department of Clinical Pathology, The Cleveland Clinic Foundation, Ohio 44195-5133, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Humans
Platelet Count / methods*
Platelet Transfusion*
Plateletpheresis / standards*,  statistics & numerical data
Reference Values

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Hoechst 33342 induces apoptosis in HL-60 cells and inhibits topoisomerase I in vivo.
Next Document:  Neuroendocrine carcinoma of the posterior mediastinum: a possible primary lesion.