Document Detail


The optimisation of parameters for the quantitative surface enhanced Raman scattering (SERS) detection of mephedrone using a fractional factorial design and a portable Raman spectrometer.
MedLine Citation:
PMID:  23198960     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A new optimisation strategy for the SERS detection of mephedrone using a portable Raman system has been developed. A fractional factorial design was employed and the number of statistically significant experiments (288) was greatly reduced from the actual total number of experiments (1722), which minimised the workload whilst maintaining the statistical integrity of the results. A number of conditions were explored in relation to mephedrone SERS signal optimisation including the type of nanoparticle, pH and aggregating agents (salts). Through exercising this design it was possible to derive the significance of each of the individual variables and we discovered four optimised SERS protocols for which the reproducibility of the SERS signal and the limit of detection (LOD) of mephedrone were established. Using traditional nanoparticles with a combination of salts and pHs it was shown that the relative standard deviations of mephedrone-specific Raman peaks were as low as 0.51% and the LOD was estimated to be around 1.6 μg/mL (9.06x10-6 M); a detection limit well beyond the scope of conventional Raman and extremely low for an analytical method optimised for quick and uncomplicated in-field use.
Authors:
Samuel Mabbott; Elon Correa; David P Cowcher; J William Allwood; Royston Goodacre
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-3
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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