| An optical microsensor to measure fluorescent light intensity in biofilms. | |
MedLine Citation:
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PMID: 15279941 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have developed an optical microsensor to quantify fluorescent light intensity distribution in biofilms. The optical system consisted of a beam splitter, light couplers, filters and a spectrophotometer able to accept the fiberoptic cable to measure fluorescent light intensity. The emitted light, fluorescence from the biofilm, was collected at the tip of the optical microsensor and was transferred to a spectrophotometer via a fiberoptic cable. The total fluorescent light intensity was evaluated from the emission spectrum by numerical integration. The newly developed fiberoptic microsensor was tested using a Staphylococcus aureus strain producing yellow fluorescent protein (YFP) grown as biofilm. We used a 405-nm violet laser diode for excitation, and measured the emission intensity between 480 nm and 540 nm. The optical microsensor that quantifies fluorescent light intensity is a promising tool in biofilm research which often requires detection and quantification of fluorescent light intensity distribution generated by various fluorescent proteins. |
Authors:
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Haluk Beyenal; Chris Yakymyshyn; Jeon Hyungnak; Catherine C Davis; Zbigniew Lewandowski |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of microbiological methods Volume: 58 ISSN: 0167-7012 ISO Abbreviation: J. Microbiol. Methods Publication Date: 2004 Sep |
Date Detail:
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Created Date: 2004-07-28 Completed Date: 2004-09-23 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8306883 Medline TA: J Microbiol Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 367-74 Citation Subset: IM |
Affiliation:
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Center for Biofilm Engineering, Montana State University, Room 366 EPS, P.O. Box 173980, Bozeman, MT 59717-3980, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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biosynthesis Biofilms / growth & development* Fiber Optic Technology / instrumentation, methods Fluorescence Luminescent Proteins / biosynthesis Spectrometry, Fluorescence / instrumentation, methods* Staphylococcus aureus / growth & development*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Luminescent Proteins; 0/yellow fluorescent protein, Bacteria |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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