Document Detail


An optical microsensor to measure fluorescent light intensity in biofilms.
MedLine Citation:
PMID:  15279941     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have developed an optical microsensor to quantify fluorescent light intensity distribution in biofilms. The optical system consisted of a beam splitter, light couplers, filters and a spectrophotometer able to accept the fiberoptic cable to measure fluorescent light intensity. The emitted light, fluorescence from the biofilm, was collected at the tip of the optical microsensor and was transferred to a spectrophotometer via a fiberoptic cable. The total fluorescent light intensity was evaluated from the emission spectrum by numerical integration. The newly developed fiberoptic microsensor was tested using a Staphylococcus aureus strain producing yellow fluorescent protein (YFP) grown as biofilm. We used a 405-nm violet laser diode for excitation, and measured the emission intensity between 480 nm and 540 nm. The optical microsensor that quantifies fluorescent light intensity is a promising tool in biofilm research which often requires detection and quantification of fluorescent light intensity distribution generated by various fluorescent proteins.
Authors:
Haluk Beyenal; Chris Yakymyshyn; Jeon Hyungnak; Catherine C Davis; Zbigniew Lewandowski
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of microbiological methods     Volume:  58     ISSN:  0167-7012     ISO Abbreviation:  J. Microbiol. Methods     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-07-28     Completed Date:  2004-09-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8306883     Medline TA:  J Microbiol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  367-74     Citation Subset:  IM    
Affiliation:
Center for Biofilm Engineering, Montana State University, Room 366 EPS, P.O. Box 173980, Bozeman, MT 59717-3980, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / biosynthesis
Biofilms / growth & development*
Fiber Optic Technology / instrumentation,  methods
Fluorescence
Luminescent Proteins / biosynthesis
Spectrometry, Fluorescence / instrumentation,  methods*
Staphylococcus aureus / growth & development*,  metabolism
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Luminescent Proteins; 0/yellow fluorescent protein, Bacteria

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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