Document Detail


The optical detection of individual DNA-conjugated gold nanoparticle labels after metal enhancement.
MedLine Citation:
PMID:  21444979     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The detection of DNA using nanoparticles as labels is an interesting alternative to the standard fluorescence technique. It requires simpler detection equipment, resulting in higher stability and lower costs. Besides easier detection, metal enhancement allows a higher sensitivity of detection. The signal-response curve for labelled DNA before and after silver enhancement was studied, applying both atomic force microscope (AFM) and optical (reflection/transmission) measurements. The dynamic range and the sensitivity were determined for nanoparticle labelling with and without metal enhancement. Nanoparticle concentrations down to the fM range could be detected. The ultimate limit of detection, the identification of individual labels, is demonstrated for the optical readout. Therefore, AFM images of the particles were correlated with the optical signal of individual or clustered particles. We demonstrate that the optical signal allows the identification of single particles.
Authors:
Andrea Csáki; Pia Kaplanek; Robert Möller; Wolfgang Fritzsche
Related Documents :
21376989 - Depth profile characterization of zn-tio(2) nanocomposite films by pulsed radiofrequenc...
22462829 - Vibrational absorption spectra from vibrational coupled cluster damped linear response ...
22243179 - Collective volume plasmons in manganites with nanoscale phase separation: simulation of...
21280629 - Controlled synthesis and size-dependent polarization domain structure of colloidal germ...
21230099 - Droplet coalescence process under electric fields in an immiscible polymer blend.
21932919 - Alignment of particles in sheared viscoelastic fluids.
24806919 - Chromatin hydrodynamics.
24769569 - Current-driven domain wall depinning from an anisotropy boundary in nanowires.
22401249 - Size dependence of exchange bias in co/coo nanostructures.
Publication Detail:
Type:  Journal Article     Date:  2003-10-22
Journal Detail:
Title:  Nanotechnology     Volume:  14     ISSN:  0957-4484     ISO Abbreviation:  Nanotechnology     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2011-03-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101241272     Medline TA:  Nanotechnology     Country:  England    
Other Details:
Languages:  eng     Pagination:  1262-8     Citation Subset:  -    
Affiliation:
Institute for Physical High Technology, PF 100239, D-07702 Jena, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Determination of energy barrier distributions of magnetic nanoparticles by temperature dependent mag...
Next Document:  The growth of freestanding single carbon nanotube arrays.