Document Detail

An optical counting technique with vertical hydrodynamic focusing for biological cells.
MedLine Citation:
PMID:  20697579     Owner:  NLM     Status:  In-Data-Review    
A BARRIER IN SCALING LABORATORY PROCESSES INTO AUTOMATED MICROFLUIDIC DEVICES HAS BEEN THE TRANSFER OF LABORATORY BASED ASSAYS: Where engineering meets biological protocol. One basic requirement is to reliably and accurately know the distribution and number of biological cells being dispensed. In this study, a novel optical counting technique to efficiently quantify the number of cells flowing into a microtube is presented. REH, B-lymphoid precursor leukemia, are stained with a fluorescent dye and frames of moving cells are recorded using a charge coupled device (CCD) camera. The basic principle is to calculate the total fluorescence intensity of the image and to divide it by the average intensity of a single cell. This method allows counting the number of cells with an uncertainty +/-5%, which compares favorably to the standard biological methodology, based on the manual Trypan Blue assay, which is destructive to the cells and presents an uncertainty in the order of 20%. The use of a microdevice for vertical hydrodynamic focusing, which can reduce the background noise of out of focus cells by concentrating the cells in a thin layer, has further improved the technique. Computational fluid dynamics (CFD) simulation and confocal laser scanning microscopy images have shown an 82% reduction in the vertical displacement of the cells. For the flow rates imposed during this study, a throughput of 100-200 cellss is achieved.
Stefano Chiavaroli; David Newport; Bernie Woulfe
Related Documents :
15472969 - Determination of the bacterial pathogen edwardsiella tarda in fish species by capillary...
12161919 - Modelling of cell proliferation: nuclear versus membrane labelling.
18816379 - A novel reverse transduction adenoviral array for the functional analysis of shrna libr...
10801039 - Fluid phase endocytosis in oral epithelia: variation with site and effect of cancer.
19304709 - Molecular imaging and quantitative measurement of epidermal growth factor receptor expr...
17161009 - Dielectrophoresis microsystem with integrated flow cytometers for on-line monitoring of...
21052479 - Surface plasmon resonance monitoring of cell monolayer integrity: implication of signal...
15472969 - Determination of the bacterial pathogen edwardsiella tarda in fish species by capillary...
2918349 - W-and y-cells in the c layers of the cat's lateral geniculate nucleus: normal propertie...
Publication Detail:
Type:  Journal Article     Date:  2010-06-15
Journal Detail:
Title:  Biomicrofluidics     Volume:  4     ISSN:  1932-1058     ISO Abbreviation:  Biomicrofluidics     Publication Date:  2010  
Date Detail:
Created Date:  2010-08-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101293825     Medline TA:  Biomicrofluidics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Rapid magnetic heating treatment by highly charged maghemite nanoparticles on Wistar rats exocranial...
Next Document:  Highly accurate deterministic lateral displacement device and its application to purification of fun...