| An optic nerve crush injury murine model to study retinal ganglion cell survival. | |
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MedLine Citation:
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PMID: 21540827 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Injury to the optic nerve can lead to axonal degeneration, followed by a gradual death of retinal ganglion cells (RGCs), which results in irreversible vision loss. Examples of such diseases in human include traumatic optic neuropathy and optic nerve degeneration in glaucoma. It is characterized by typical changes in the optic nerve head, progressive optic nerve degeneration, and loss of retinal ganglion cells, if uncontrolled, leading to vision loss and blindness. The optic nerve crush (ONC) injury mouse model is an important experimental disease model for traumatic optic neuropathy, glaucoma, etc. In this model, the crush injury to the optic nerve leads to gradual retinal ganglion cells apoptosis. This disease model can be used to study the general processes and mechanisms of neuronal death and survival, which is essential for the development of therapeutic measures. In addition, pharmacological and molecular approaches can be used in this model to identify and test potential therapeutic reagents to treat different types of optic neuropathy. Here, we provide a step by step demonstration of (I) Baseline retrograde labeling of retinal ganglion cells (RGCs) at day 1, (II) Optic nerve crush injury at day 4, (III) Harvest the retinae and analyze RGC survival at day 11, and (IV) Representative result. |
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Authors:
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Zhongshu Tang; Shuihua Zhang; Chunsik Lee; Anil Kumar; Pachiappan Arjunan; Yang Li; Fan Zhang; Xuri Li |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Intramural; Video-Audio Media Date: 2011-04-25 |
Journal Detail:
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Title: Journal of visualized experiments : JoVE Volume: - ISSN: 1940-087X ISO Abbreviation: J Vis Exp Publication Date: 2011 |
Date Detail:
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Created Date: 2011-05-04 Completed Date: 2011-08-19 Revised Date: 2013-05-24 |
Medline Journal Info:
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Nlm Unique ID: 101313252 Medline TA: J Vis Exp Country: United States |
Other Details:
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Languages: eng Pagination: - Citation Subset: IM |
Affiliation:
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National Eye Institute, NIH, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Survival / physiology Disease Models, Animal Fluorescent Dyes / chemistry Mice Microscopy, Fluorescence / methods Optic Nerve / pathology* Optic Nerve Injuries / pathology* Retinal Ganglion Cells / pathology* Staining and Labeling / methods |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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