Document Detail


The opsonic activity of stored blood.
MedLine Citation:
PMID:  405758     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Experiments were performed to measure the stability of opsonins during storage of whole blood collected in CPD solution and stored at 4 C. Three units were collected, and aliquots removed and recalcified on days 0, 3, 7, 14, 21, and 28. Reconstituted serum was immediately frozen and stored at -70 C. The ability to opsonize Escherichia coli 075 and Pseudomonas aeruginosa immunotype 1, both of which require activation and utilization of the alternative pathway of complement for opsonization, were performed simultaneously for each organism and for each unit. Results showed that there was a slight but insignificant reduction in opsonic strength during storage for up to 28 days. The results indicate that standard collection and storage conditions using CPD as the anticoagulant retains functional activity of the alternative pathway. C3 measured by radial immunodiffusion using antisera against the B antigenic determinant showed that C3 had not been converted during storage.
Authors:
M A McClellan; J W Alexander
Related Documents :
3219108 - Cramps following exercise.
3753358 - Effect of hypoxia on heart glycogen utilization during exercise.
3693208 - Effects of gluconeogenic precursor flux alterations on glycogen resynthesis after prolo...
3685148 - The effects of age on substrate depletion and hormonal responses during submaximal exer...
21885518 - The omeract ultrasound task force -- status and perspectives.
16893708 - Usefulness of n-terminal pro-b-type natriuretic peptide levels to predict exercise capa...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transfusion     Volume:  17     ISSN:  0041-1132     ISO Abbreviation:  Transfusion     Publication Date:    1977 May-Jun
Date Detail:
Created Date:  1977-07-23     Completed Date:  1977-07-23     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  227-32     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Blood Preservation*
Complement C3 / metabolism
Complement System Proteins
Escherichia coli / immunology
Humans
Opsonin Proteins*
Phagocytosis
Pseudomonas aeruginosa / immunology
Chemical
Reg. No./Substance:
0/Complement C3; 0/Opsonin Proteins; 9007-36-7/Complement System Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Estimation of cell damage in monolayer culture using 14C-leucine incorporation.
Next Document:  Cytotoxic effects of normal sera on lymphoid cells. II. Requirements for inhibition of nonspecific s...