Document Detail

The opsonic activity of stored blood.
MedLine Citation:
PMID:  405758     Owner:  NLM     Status:  MEDLINE    
Experiments were performed to measure the stability of opsonins during storage of whole blood collected in CPD solution and stored at 4 C. Three units were collected, and aliquots removed and recalcified on days 0, 3, 7, 14, 21, and 28. Reconstituted serum was immediately frozen and stored at -70 C. The ability to opsonize Escherichia coli 075 and Pseudomonas aeruginosa immunotype 1, both of which require activation and utilization of the alternative pathway of complement for opsonization, were performed simultaneously for each organism and for each unit. Results showed that there was a slight but insignificant reduction in opsonic strength during storage for up to 28 days. The results indicate that standard collection and storage conditions using CPD as the anticoagulant retains functional activity of the alternative pathway. C3 measured by radial immunodiffusion using antisera against the B antigenic determinant showed that C3 had not been converted during storage.
M A McClellan; J W Alexander
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transfusion     Volume:  17     ISSN:  0041-1132     ISO Abbreviation:  Transfusion     Publication Date:    1977 May-Jun
Date Detail:
Created Date:  1977-07-23     Completed Date:  1977-07-23     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  227-32     Citation Subset:  IM    
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MeSH Terms
Blood Preservation*
Complement C3 / metabolism
Complement System Proteins
Escherichia coli / immunology
Opsonin Proteins*
Pseudomonas aeruginosa / immunology
Reg. No./Substance:
0/Complement C3; 0/Opsonin Proteins; 9007-36-7/Complement System Proteins

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