Document Detail


The ontogeny of the glucose-6-phosphatase enzyme in human embryonic and fetal red blood cells.
MedLine Citation:
PMID:  7588160     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have shown for the first time that the microsomal glucose-6-phosphatase enzyme protein is present in human embryonic and fetal red blood cells and the ontogeny of its expression has been determined. In the earliest embryos, red cells are predominantly of the primitive megaloblastic type. Circulating red cells in the primitive megaloblastic series are predominantly nucleated and glucose-6-phosphatase immunopositive. Non-nucleated, immunoreactive megaloblastic cells are in a minority. In fetuses > 12 weeks gestation, the erythrocytes are of the definitive normoblastic series and in the transitional period of switch-over in late embryonic-early fetal life, up to 30% of glucose-6-phosphatase immunopositive cells are definitive normoblastic in type, with a variable contribution from nucleated and non-nucleated cells. Thereafter, the number of immunopositive cells in the definitive normoblastic series decreases such that after 12 weeks gestation it is less than 5%. The fact that a predominantly hepatic protein in adults (glucose-6-phosphatase) is present in embryonic and fetal red blood cells, particularly nucleated red cells, raises the possibility of diagnosis of disorders of liver protein expression in nucleated fetal red cells isolated from the first trimester maternal circulation.
Authors:
R Hume; S Pazouki; A Hallas; A Burchell
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Early human development     Volume:  42     ISSN:  0378-3782     ISO Abbreviation:  Early Hum. Dev.     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-12-15     Completed Date:  1995-12-15     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  7708381     Medline TA:  Early Hum Dev     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  85-95     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynaecology, University of Dundee, Ninewells Hospital and Medical School, Scotland, UK.
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MeSH Terms
Descriptor/Qualifier:
Embryo, Mammalian / cytology,  enzymology*
Erythrocyte Count
Erythrocytes / enzymology*
Female
Fetal Blood / cytology,  enzymology*
Gestational Age
Glucose-6-Phosphatase / blood*,  metabolism
Humans
Immunohistochemistry
Pregnancy
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
EC 3.1.3.9/Glucose-6-Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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